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Literature summary for 3.1.1.3 extracted from

  • Namboodiri, V.M.H.; Chattopadhyaya, R.
    Purification and biochemical characterization of a novel thermostable lipase from Aspergillus niger (2000), Lipids, 35, 495-502.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inhibition at 10-30 mM Aspergillus niger
chymotrypsin proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
Cu2+ strong inhibition, 40% residual activity at 50 mM Aspergillus niger
endoprotease Glu-C proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
Fe2+ strong inhibition, 30% residual activity at 50 mM Aspergillus niger
Fe3+ strong inhibition, 25% residual activity at 50 mM Aspergillus niger
additional information no inhibition by EDTA 50 mM Aspergillus niger
papain proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
thrombin proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
Trypsin proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme activity is not affected by Mg2+, K+, Na+, Ca2+, Mn2+, NH4+, and Zn2+ up to 1.0 M Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 2.8% sugar, which is not essentail for activity Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
50fold, to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
208.2
-
purified enzyme Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate is olive oil, the enzyme is 1,3-specific Aspergillus niger ?
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 55
-
Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
60 min stable Aspergillus niger
60
-
50% remaining activity after 60 min Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5
-
Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing
-
4.4