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Literature summary for 3.1.1.3 extracted from
- Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 (2002), Eur. J. Biochem., 269, 5771-5779.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Acinetobacter sp. |  |
| Co2+ | - |
Acinetobacter sp. | |
| Fe3+ | - |
Acinetobacter sp. | |
| Mg2+ | slight activation | Acinetobacter sp. | |
| Mn2+ | slight activation | Acinetobacter sp. | |
| Rb+ | - |
Acinetobacter sp. | |
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes the enzyme, no loss of activity after 30 h at 30°C in presence of Ca2+, 70% loss after 30 h at 30°C of activity in absence of Ca2+ | Acinetobacter sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong inhibition | Acinetobacter sp. | |
| EDTA | strong, about 90% inhibition at 1 mM | Acinetobacter sp. | |
| Hg2+ | strong inhibition | Acinetobacter sp. | |
| additional information | no inhibition by 2-mercaptoethanol, dithiothreitol, and Zn2+ | Acinetobacter sp. | |
| PMSF | slight inhibition | Acinetobacter sp. | |
| Pyridine | rapid deactivation | Acinetobacter sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | LipA, secreted to the medium | Acinetobacter sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Acinetobacter sp. |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetonitrile | loss of about 25% activity at 30% v/v after 1 h at 30°C | Acinetobacter sp. |
| dimethylformamide | loss of about 10% activity at 30% v/v after 1 h at 30°C | Acinetobacter sp. |
| additional information | the enzyme is only very slightly affected by isopropanol and acetone | Acinetobacter sp. |
| Pyridine | rapid complete deactivation | Acinetobacter sp. |
| tetrahydrofuran | loss of about 90% activity at 30% v/v after 1 h at 30°C | Acinetobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter sp. | - |
LipA | - |
| Acinetobacter sp. RAG-1 | - |
LipA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from culture medium, 10.7fold | Acinetobacter sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 412 | - |
purified enzyme | Acinetobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | low activity | Acinetobacter sp. | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | low activity | Acinetobacter sp. RAG-1 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Acinetobacter sp. RAG-1 | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl decanoate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | best substrate | Acinetobacter sp. | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | best substrate | Acinetobacter sp. RAG-1 | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl myristate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + myristate | - |
? | |
| 4-nitrophenyl octanoate + H2O | best substrate | Acinetobacter sp. | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | best substrate | Acinetobacter sp. RAG-1 | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + stearate | - |
? | |
| additional information | substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids | Acinetobacter sp. | ? | - |
? | |
| additional information | substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids | Acinetobacter sp. RAG-1 | ? | - |
? | |
| tributyrin + H2O | - |
Acinetobacter sp. | dibutyrin + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Acinetobacter sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LipA | - |
Acinetobacter sp. |
| lipase | - |
Acinetobacter sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Acinetobacter sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 33% of maximal activity at 30°C, about 50% of maximal activity at 70°C | Acinetobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
active up to | Acinetobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Acinetobacter sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | high activity | Acinetobacter sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Acinetobacter sp. | isoelectric focusing | - |
5.9 |
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