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Literature summary for 3.1.1.3 extracted from

  • Maruyama, T.; Nakajima, M.; Kondo, H.; Kawasaki, K.; Seki, M.; Goto, M.
    Can lipases hydrolyze a peptide bond? (2003), Enzyme Microb. Technol., 32, 655-657.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene lipA, expression in Bacillus megaterium WH320 Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to the medium, native and recombinant enzyme Bacillus subtilis
-
-

Organism

Organism UniProt Comment Textmining
Alcaligenes ssp.
-
commercial product
-
Bacillus subtilis
-
gene lipA
-
Bacillus subtilis 168 BsL
-
gene lipA
-
Chromobacterium viscosum
-
commercial product
-
Diutina rugosa
-
type VII, commercial product
-
Fusarium solani
-
cutinase, commercial product, 90% pure enzyme
-
Homo sapiens
-
-
-
Rhizomucor miehei
-
commercial product
-
Rhizopus arrhizus
-
commercial product
-
Rhizopus japonicus
-
commercial product
-
Sus scrofa
-
3 commercially available enzymes, PPL
-
Thermomyces lanuginosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of the commercial product from elastin Sus scrofa
recombinant extracellular enzyme, expressed in Bacillus megaterium, from culture supernatant, to more than 90% purity Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Homo sapiens
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Sus scrofa
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Rhizopus arrhizus
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Diutina rugosa
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Thermomyces lanuginosus
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Rhizopus japonicus
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Chromobacterium viscosum
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases Alcaligenes ssp.
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases, enzyme contains the alpha-helical lid structure Rhizomucor miehei
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases, lipase contains no lid structure Fusarium solani
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases, the enzyme contains no lid structure Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
cartilage elastin, commercial product Sus scrofa
-
pancreas
-
Homo sapiens
-
pancreas 2 commercially available enzymes Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenol butyrate + H2O
-
Bacillus subtilis 4-nitrophenol + butyrate
-
?
4-nitrophenol butyrate + H2O
-
Bacillus subtilis 168 BsL 4-nitrophenol + butyrate
-
?
additional information enzyme also utilizes water-soluble substrates, enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Fusarium solani ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Bacillus subtilis ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Homo sapiens ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Sus scrofa ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Rhizopus arrhizus ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Diutina rugosa ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Rhizomucor miehei ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Thermomyces lanuginosus ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Rhizopus japonicus ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Chromobacterium viscosum ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Alcaligenes ssp. ?
-
?
additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Bacillus subtilis 168 BsL ?
-
?
tributyrin + H2O
-
Bacillus subtilis dibutyrin + butyrate
-
?
tributyrin + H2O
-
Homo sapiens dibutyrin + butyrate
-
?
tributyrin + H2O
-
Sus scrofa dibutyrin + butyrate
-
?
tributyrin + H2O
-
Rhizopus arrhizus dibutyrin + butyrate
-
?
tributyrin + H2O
-
Fusarium solani dibutyrin + butyrate
-
?
tributyrin + H2O
-
Diutina rugosa dibutyrin + butyrate
-
?
tributyrin + H2O
-
Rhizomucor miehei dibutyrin + butyrate
-
?
tributyrin + H2O
-
Thermomyces lanuginosus dibutyrin + butyrate
-
?
tributyrin + H2O
-
Rhizopus japonicus dibutyrin + butyrate
-
?
tributyrin + H2O
-
Chromobacterium viscosum dibutyrin + butyrate
-
?
tributyrin + H2O
-
Alcaligenes ssp. dibutyrin + butyrate
-
?
tributyrin + H2O
-
Bacillus subtilis 168 BsL dibutyrin + butyrate
-
?

Synonyms

Synonyms Comment Organism
cutinase
-
Fusarium solani
lipase
-
Bacillus subtilis
lipase
-
Homo sapiens
lipase
-
Sus scrofa
lipase
-
Rhizopus arrhizus
lipase
-
Diutina rugosa
lipase
-
Rhizomucor miehei
lipase
-
Thermomyces lanuginosus
lipase
-
Rhizopus japonicus
lipase
-
Chromobacterium viscosum
lipase
-
Alcaligenes ssp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bacillus subtilis
25
-
assay at Homo sapiens
25
-
assay at Sus scrofa
25
-
assay at Rhizopus arrhizus
25
-
assay at Fusarium solani
25
-
assay at Diutina rugosa
25
-
assay at Rhizomucor miehei
25
-
assay at Thermomyces lanuginosus
25
-
assay at Rhizopus japonicus
25
-
assay at Chromobacterium viscosum
25
-
assay at Alcaligenes ssp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bacillus subtilis
7
-
assay at Homo sapiens
7
-
assay at Sus scrofa
7
-
assay at Rhizopus arrhizus
7
-
assay at Fusarium solani
7
-
assay at Diutina rugosa
7
-
assay at Rhizomucor miehei
7
-
assay at Thermomyces lanuginosus
7
-
assay at Rhizopus japonicus
7
-
assay at Chromobacterium viscosum
7
-
assay at Alcaligenes ssp.