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Literature summary for 3.1.1.3 extracted from

  • Lee, D.W.; Kim, H.W.; Lee, K.W.; Kim, B.C.; Choe, E.A.; Lee, H.S.; Kim, D.S.; Pyun, Y.R.
    Purification and characterization of two distinct thermostable lipases from the gram-positive thermophilic bacterium Bacillus thermoleovorans ID-1 (2001), Enzyme Microb. Technol., 29, 363-371.
No PubMed abstract available
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ BTID-B, slight activation Geobacillus thermoleovorans
Co2+ BTID-B, slight activation Geobacillus thermoleovorans
K+ BTID-B, slight activation Geobacillus thermoleovorans
Mn2+ BTID-B, slight activation Geobacillus thermoleovorans
Na+ BTID-B, slight activation Geobacillus thermoleovorans

Cloned(Commentary)

Cloned (Comment) Organism
BTID-B, DNA sequence determination and analysis, expression in Escherichia coli Geobacillus thermoleovorans

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol BTID-A, 26% inhibition at 1% w/v Geobacillus thermoleovorans
Ag+ BTID-A Geobacillus thermoleovorans
Ca2+ BTID-A, slight inhibition Geobacillus thermoleovorans
Co2+ BTID-A Geobacillus thermoleovorans
Cu2+ BTID-A, BTID-B Geobacillus thermoleovorans
dithiothreitol BTID-A, 48% inhibition at 1% w/v Geobacillus thermoleovorans
DMSO BTID-A, 54% inhibition at 1% w/v Geobacillus thermoleovorans
EDTA BTID-B, 45% inhibition at 1% w/v, BTID-A, 68% inhibition at 1% w/v Geobacillus thermoleovorans
ethanol BTID-A, 42% inhibition at 1% w/v Geobacillus thermoleovorans
Fe2+ BTID-A, BTID-B Geobacillus thermoleovorans
Hg2+ BTID-A, BTID-B Geobacillus thermoleovorans
K+ BTID-A Geobacillus thermoleovorans
Mg2+ BTID-A Geobacillus thermoleovorans
Mn2+ BTID-A Geobacillus thermoleovorans
Na+ BTID-A Geobacillus thermoleovorans
PMSF BTID-A, 92% inhibition at 1% w/v Geobacillus thermoleovorans
SDS BTID-B, complete inhibition at 1% w/v, no inhibition of BTID-A Geobacillus thermoleovorans
Zn2+ BTID-A, BTID-B Geobacillus thermoleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.82
-
 tricaprylin BTID-A, pH 7.5, 60°C Geobacillus thermoleovorans
6.24
-
 tricaprylin BTID-B, pH 7.5, 60°C Geobacillus thermoleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 x * 18000, BTID-A, SDS-PAGE, 1* 43000, BTID-B, SDS-PAGE Geobacillus thermoleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Geobacillus thermoleovorans enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources ?
-
 ?
additional information Geobacillus thermoleovorans ID-1 enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information BTID-B is strongly resistant to organic solvents Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans
-
 2 thermostable lipases A and B, BTID-A and BTID-B
-
Geobacillus thermoleovorans ID-1
-
 2 thermostable lipases A and B, BTID-A and BTID-B
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, BTID-A 300fold, recombinant BTID-B 107.5fold Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 purified BTID-A Geobacillus thermoleovorans
292
-
 purified recombinant BTID-B Geobacillus thermoleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3-trioleoyl-glycerol + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1,2-dilinoleoyl-3-oleoyl-rac-glycerol + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1,3-dioleoyl-2-palmitoyl-glycerol + H2O not BTID-A Geobacillus thermoleovorans ?
-
 ?
1,3-dipalmitoyl-2-oleoyl-glycerol + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
additional information enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources Geobacillus thermoleovorans ?
-
 ?
additional information enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources Geobacillus thermoleovorans ID-1 ?
-
 ?
tributyrin + H2O not BTID-A Geobacillus thermoleovorans dibutyrin + butyrate
-
 ?
tributyrin + H2O not BTID-A Geobacillus thermoleovorans ID-1 dibutyrin + butyrate
-
 ?
tricaprin + H2O
-
 Geobacillus thermoleovorans dicaprin + decaprate
-
 ?
tricaproin + H2O not BTID-A Geobacillus thermoleovorans dicaproin + caproate
-
 ?
tricaprylin + H2O best substrate, BTID-A and BTID-B Geobacillus thermoleovorans dicaprylin + caprylate
-
 ?
tricaprylin + H2O best substrate, BTID-A and BTID-B Geobacillus thermoleovorans ID-1 dicaprylin + caprylate
-
 ?
trilaurin + H2O
-
 Geobacillus thermoleovorans dilaurin + laurate
-
 ?
trilaurin + H2O
-
 Geobacillus thermoleovorans ID-1 dilaurin + laurate
-
 ?
trimyristin + H2O
-
 Geobacillus thermoleovorans dimyristin + myristate
-
 ?
tripalmitin + H2O
-
 Geobacillus thermoleovorans dipalmitin + palmitate
-
 ?
tripalmitin + H2O
-
 Geobacillus thermoleovorans ID-1 dipalmitin + palmitate
-
 ?
tristearin + H2O
-
 Geobacillus thermoleovorans distearin + stearate
-
 ?

Subunits

Subunits Comment Organism
? x * 18000, BTID-A, SDS-PAGE, 1* 43000, BTID-B, SDS-PAGE Geobacillus thermoleovorans

Synonyms

Synonyms Comment Organism
BTID
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 BTID-B Geobacillus thermoleovorans
60 65 BTID-A Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 BTID-B is stable for 2 h, BTID-A shows over 75% remaining activity after 30 min Geobacillus thermoleovorans
70
-
 BTID-B, 30 min, 50% remaining activity Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 BTID-B Geobacillus thermoleovorans
9
-
 BTID-A Geobacillus thermoleovorans