Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutants lacking the cap and the lid domain via baculovirus transfection in insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutants lacking the cap and the lid domain by oligonucleotide-directed mutagenesis, influencing the expresion level, stability, activity, and reaction kinetics | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion in the stomach | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE | Homo sapiens |
43000 | - |
x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE | Homo sapiens |
47000 | - |
x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gastric lipase | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from insect cells, mutant lacking the cap domain is only stable at acidic pH value during purification | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | catalytic serine residue, deeply buried under a domain called the extrusion domain, which is composed of a cap and a lid segmentof 58 amino acids, catalytic reaction mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
purified recombinant mutant enzyme lacking the cap domain, substrate triolein | Homo sapiens |
0.5 | - |
purified recombinant mutant enzyme lacking the lid domain, substrate triolein | Homo sapiens |
30 | - |
purified recombinant wild-type enzyme, substrate triolein | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
triolein + H2O | - |
Homo sapiens | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE | Homo sapiens |
More | epitope mapping | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HGL | - |
Homo sapiens |
More | the enzyme belongs to the alpha/beta hydrolase fold family | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Homo sapiens |