Cloned (Comment) | Organism |
---|---|
subcloning of plasmids encoding mutant enzymes in Escherichia coli XLIB, expression of mutants in Pichia pastoris GS115 | Rhizomucor miehei |
Protein Variants | Comment | Organism |
---|---|---|
F94E | site-directed mutagenesis, altered substrate chain length specificity | Rhizomucor miehei |
F94Q | site-directed mutagenesis, altered substrate chain length specificity | Rhizomucor miehei |
F94R | site-directed mutagenesis, altered substrate chain length specificity | Rhizomucor miehei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
preparative level, recombinant mutants expressed in Pichia pastoris | Rhizomucor miehei |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | Phe94 is involved in substrate binding and is responsible for accommodating the acyl chain length of the substrate | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-O-dilauryl-rac-glycero-3-glutaric acid resorufin ester + H2O | - |
Rhizomucor miehei | ? | - |
? | |
4-nitrophenyl butyrate + H2O | low activity | Rhizomucor miehei | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Rhizomucor miehei | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | low activity | Rhizomucor miehei | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | low activity | Rhizomucor miehei | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl tetradecanoate + H2O | best 4-nitrophenyl ester substrate for wild-type enzyme and mutant F94R | Rhizomucor miehei | 4-nitrophenol + tetradecanoate | - |
? | |
additional information | substrate chain length specificity of wild-type and mutant enzymes | Rhizomucor miehei | ? | - |
? | |
tributyrin + H2O | best triacylglyceride substrate for the wild-type enzyme | Rhizomucor miehei | dibutyrin + butyrate | - |
? | |
tridecanin + H2O | - |
Rhizomucor miehei | didecanin + decanoate | - |
? | |
trihexanin + H2O | - |
Rhizomucor miehei | dihexanin + hexanoate | - |
? | |
trioctanin + H2O | - |
Rhizomucor miehei | dioctanin + octanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Rhizomucor miehei |