Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, expression in Escherichia coli XL1-Blue and in Bacillus subtilis DB104 | Bacillus pumilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to the culture medium | Bacillus pumilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme shows Ca2+-independent thermostability and catalytic activity, enzyme contains no Ca2+-binding motif around the catalytic His156 residue | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
Ca2+-independent lipase | - |
Bacillus pumilus B26 | - |
Ca2+-independent lipase | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Bacillus subtilis culture supernatant, 4.3fold, purification of the wild-type Bacillus pumilus strain is not possible due to the low expression level | Bacillus pumilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
138 | - |
purified enzyme, substrate is olive oil emulsion | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | the enzyme shows a typical interfacial activation mechanism towards the substrate | Bacillus pumilus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | the enzyme shows a typical interfacial activation mechanism towards the substrate | Bacillus pumilus B26 | 4-nitrophenol + butyrate | - |
? | |
additional information | an emulsion containing olive oil and gum arabic is used as substrate, enzyme shows a preference for short-chain triacylglycerides | Bacillus pumilus | ? | - |
? | |
additional information | an emulsion containing olive oil and gum arabic is used as substrate, enzyme shows a preference for short-chain triacylglycerides | Bacillus pumilus B26 | ? | - |
? | |
tributyrin + H2O | - |
Bacillus pumilus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Bacillus pumilus B26 | dibutyrin + butyrate | - |
? | |
triolein + H2O | low activity | Bacillus pumilus | diolein + oleate | - |
? | |
triolein + H2O | low activity | Bacillus pumilus B26 | diolein + oleate | - |
? | |
tripropionin + H2O | the enzyme shows a typical interfacial activation mechanism towarsd the substrate | Bacillus pumilus | dipropionin + propionate | - |
? | |
tripropionin + H2O | the enzyme shows a typical interfacial activation mechanism towarsd the substrate | Bacillus pumilus B26 | dipropionin + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase B26 | - |
Bacillus pumilus |
More | the enzyme belongs to the lipase family I.4 | Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to, in presence or absence of 10 mM Ca2+ | Bacillus pumilus |
45 | - |
rapid decrease in activity | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus pumilus |