Literature summary for 3.1.1.29 extracted from

Singh, A.; Kumar, A.; Gautam, L.; Sharma, P.; Sinha, M.; Bhushan, A.; Kaur, P.; Sharma, S.; Arora, A.; Singh, T.P.
Structural and binding studies of peptidyl-tRNA hydrolase from Pseudomonas aeruginosa provide a platform for the structure based inhibitor design against peptidyl-tRNA hydrolase (2014), Biochem. J., 463, 329-337.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(lambdaDE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
native enzyme and in complex with 3'-deoxy-N[(O-methyl-L-tyrosyl)amino]adenosine or 5-azacytidine, hanging drop vapor diffusion method, using 25% (w/v) PEG 4000, 5% (v/v) propan-2-ol and 0.1 M HEPES buffer, pH 7.5	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
3'-deoxy-N[(O-methyl-L-tyrosyl)amino]adenosine	-	Pseudomonas aeruginosa
5-azacytidine	-	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, SDS-PAGE	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HVC3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex G-75 gel filtration	Pseudomonas aeruginosa

Subunits

Subunits	Comment	Organism
?	x * 24000, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
peptidyl-tRNA hydrolase	-	Pseudomonas aeruginosa
PTH	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)