Literature summary for 3.1.1.29 extracted from

Vandavasi, V.; Taylor-Creel, K.; McFeeters, R.L.; Coates, L.; McFeeters, H.
Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium (2014), Acta Crystallogr. Sect. F, 70, 872-877.

Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 pLysS cells	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.03 M citric acid, 0.05 M Bis-Tris propane, 1% (v/v) glycerol, 3% (w/v) sucrose, 25% (w/v) PEG 6000 pH 7.6	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	x * 25000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B281	-	-
Salmonella enterica subsp. enterica serovar Typhimurium 14028s	A0A0F6B281	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-chelating affinity column chromatography and Superose 12 gel filtration	Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability	Organism
22°C, 20 mM Bis-Tris pH 6.6, 50 mM NaCl, 2 mM dithiothreitol, several weeks, without loss of activity or precipitation	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptidyl-tRNA + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?
peptidyl-tRNA + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium 14028s	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 25000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
peptidyl-tRNA hydrolase	-	Salmonella enterica subsp. enterica serovar Typhimurium
Pth1	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)