Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method at 20°C, crystal structure is determined at 2.7 A resolution | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
C703A | mutation abolishes editing activity | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc ion is coordinated by the conserved zinc-binding cluster in the C-domain, which is expected to be the enzymatic active site | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
gel filtration | Pyrococcus horikoshii |
25300 | - |
1 * 25300, calculated from sequence | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O57848 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-tRNAAla + H2O | - |
Pyrococcus horikoshii | Gly + tRNAAla | - |
? | |
Ser-tRNAAla + H2O | - |
Pyrococcus horikoshii | Ser + tRNAAla | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25300, calculated from sequence | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
alanyl-tRNA editing protein AlaX-M | - |
Pyrococcus horikoshii |
AlaX-M trans-editing enzyme | - |
Pyrococcus horikoshii |
PH0108 | - |
Pyrococcus horikoshii |