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Literature summary for 3.1.1.29 extracted from

  • Richter, R.; Rorbach, J.; Pajak, A.; Smith, P.M.; Wessels, H.J.; Huynen, M.A.; Smeitink, J.A.; Lightowlers, R.N.; Chrzanowska-Lightowlers, Z.M.
    A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome (2010), EMBO J., 29, 1116-1125.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed as GST-fusion protein in Escherichia coli Rosetta pLysS (GST later cleaved), or in human HEK293T cells as FLAG-tagged protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information truncated ICT1 form lacking the N-terminal 29 residues leads to reduction in mitochondrial protein synthesis, a mutation of the GGQ domain of ICT1 by site-directed mutagenesis causes loss of cell viability Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion mitochondiral ribosome Homo sapiens 5739
-
ribosome mitochondiral ribosome Homo sapiens 5840
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q14197 colon carcinoma HeLa cells
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
ICT1 immature colon carcinoma transcript-1 with PTH activity Homo sapiens
peptidyl-tRNA hydrolase
-
 Homo sapiens
PTH
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function ICT1 is a member of the large mitoribosomal subunit: it behaves as an integral member of the 39S mt-LSU and a component of the intact 55S monosome Homo sapiens