Application | Comment | Organism |
---|---|---|
drug development | Pth is a potential drug target to control eubacterial infections | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | genetic interactions and the mechanism of peptidyl-tRNA drop-off of translating ribosomes leading to accumutaion of peptidyl-tRNA, overview | ? | - |
? | |
peptidyl-tRNAL + H2O | Escherichia coli | Pth is a key protein at the crossroads to the function of several translational factors, accumulation of peptidyl-tRNA in the cells leads to depletion of aminoacyl-tRNA pools and halts protein biosynthesis, it is vital for cells to maintain Pth activity to deal with the pollution of peptidyl-tRNAs generated during the initiation, elongation and termination steps of protein biosynthesis, overview | peptide + tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant strain with temperature-sensitive Pth, gene pth | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA | reaction mechanism, active-site residues N10, H20 and D93 are crucial for catalysis | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | genetic interactions and the mechanism of peptidyl-tRNA drop-off of translating ribosomes leading to accumutaion of peptidyl-tRNA, overview | Escherichia coli | ? | - |
? | |
peptidyl-tRNAL + H2O | Pth is a key protein at the crossroads to the function of several translational factors, accumulation of peptidyl-tRNA in the cells leads to depletion of aminoacyl-tRNA pools and halts protein biosynthesis, it is vital for cells to maintain Pth activity to deal with the pollution of peptidyl-tRNAs generated during the initiation, elongation and termination steps of protein biosynthesis, overview | Escherichia coli | peptide + tRNA | - |
? | |
peptidyl-tRNAL + H2O | Pth prefers substrates with two or more peptide bonds compared to those with a single peptide bond | Escherichia coli | peptide + tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidyl-tRNA hydrolase | - |
Escherichia coli |
PTH | - |
Escherichia coli |