Cloned (Comment) | Organism |
---|---|
gene pth, orf Rv1014c, DNA and amino acid sequence determination, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, microbatch method, 0.002 ml each of the protein solution, containing 6 mg/ml protein, 20 mM Tris-HCl, pH 7.5, 100 mM NaCl and 2 mM 2-mercaptoethanol, and the precipitant solution, containing 20% w/v PEG 8000 in 0.1 M HEPES, pH 7.5, and 5% v/v 2-propanol or dioxane or 25% w/v PEG 8000, 100 mM sodium cacodylate, pH 6.6, and 5% v/v 2-propanol, are mixed, 5 days to 2 weeks, X-ray diffraction structure determination and analysis at 1.97-2.49 A resolution | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20455 | - |
x * 20455, sequence calculation | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene pth, orf Rv1014c | - |
Mycobacterium tuberculosis H37Rv | - |
gene pth, orf Rv1014c | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme salvages tRNA from peptidyl-tRNA by hydrolyzing the ester link between the peptide and the 2'-or 3'-OH of the sugar at the end of tRNA, since accumulation of peptidyl-tRNA, due to drop-off of translating ribosomes, is toxic to the cell, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20455, sequence calculation | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
peptidyl-tRNA hydrolase | - |
Mycobacterium tuberculosis |
PTH | - |
Mycobacterium tuberculosis |