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Literature summary for 3.1.1.29 extracted from

  • Ferreiro, C.; Heredia, C.F.
    Purification and characterization of an aminoacyl-tRNA hydrolase from the filamentous fungus Fusarium culmorum (1986), Biochim. Biophys. Acta, 882, 410-418.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
deacetylated tRNA
-
Fusarium culmorum
EDTA 5 mM, complete inhibition Fusarium culmorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
N-acetyl-Phe-tRNA pH 7.5, 30°C Fusarium culmorum
0.0008
-
Phe-tRNA pH 7.5, 30°C Fusarium culmorum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation is 25% lower than with Mg2+ or Mn2+ Fusarium culmorum
Mg2+ maximal activity is obtained with 20 mM Mg2+ or Mn2+ Fusarium culmorum
Mn2+ maximal activity is obtained with 20 mM Mg2+ or Mn2+ Fusarium culmorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
gel filtration Fusarium culmorum

Organism

Organism UniProt Comment Textmining
Fusarium culmorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Fusarium culmorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0051
-
-
Fusarium culmorum

Storage Stability

Storage Stability Organism
-10°C, in presence of 1 mg/ml bovine serum albumin, no loss of activity Fusarium culmorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-tRNA + H2O
-
Fusarium culmorum Ala + tRNA
-
?
Leu-tRNA + H2O
-
Fusarium culmorum Leu + tRNA
-
?
Lys-tRNA + H2O
-
Fusarium culmorum Lys + tRNA
-
?
Met-tRNA + H2O
-
Fusarium culmorum Met + tRNA
-
?
additional information enzyme shows species specificity. Aminoacyl-tRNAs in which the tRNA comes from eucaryotes are equally efficient as substrates of the enzyme, when tRNA comes from procaryotic organisms it is hydrolyzed 40% less efficiently Fusarium culmorum ?
-
?
N-acetyl-Ala-tRNA + H2O
-
Fusarium culmorum N-acetyl-Ala + tRNA
-
?
N-acetyl-Leu-tRNA + H2O
-
Fusarium culmorum N-acetyl-Leu + tRNA
-
?
N-acetyl-Lys-tRNA + H2O
-
Fusarium culmorum N-acetyl-Lys + tRNA
-
?
N-acetyl-Met-tRNA + H2O
-
Fusarium culmorum N-acetyl-Met + tRNA
-
?
N-acetyl-Phe-tRNA + H2O
-
Fusarium culmorum N-acetyl-Phe + tRNA
-
?
N-acetyl-Ser-tRNA + H2O
-
Fusarium culmorum N-acetyl-Ser + tRNA
-
?
N-acetyl-Tyr-tRNA + H2O
-
Fusarium culmorum N-acetyl-Tyr + tRNA
-
?
N-acetyl-Val-tRNA + H2O
-
Fusarium culmorum N-acetyl-Val + tRNA
-
?
Phe-tRNA + H2O
-
Fusarium culmorum Phe + tRNA
-
?
Ser-tRNA + H2O
-
Fusarium culmorum Ser + tRNA
-
?
Tyr-tRNA + H2O
-
Fusarium culmorum Tyr + tRNA
-
?
Val-tRNA + H2O
-
Fusarium culmorum Val + tRNA
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, 70% loss of activity Fusarium culmorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Fusarium culmorum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 30°C, 30 min, stable Fusarium culmorum