KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2-(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
- |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
A.312 | - |
Pseudomonas putida A.312 | - |
A.312 | - |
Purification (Comment) | Organism |
---|---|
422fold | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
717 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Pseudomonas putida | 3-oxoadipate | - |
? | |
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Pseudomonas putida A.312 | 3-oxoadipate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoadipate enol-lactone hydrolase | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life, 3 min in 0.02 M sodium potassium phosphate buffer, pH 6.8, 0.005 mM Mg-EDTA | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | decrease of the activity is dependent on the buffer, the enzyme is less active in Tris-HCl than in potassium phosphate buffer at pH 7.5 | Pseudomonas putida |