Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | 0.5 mM, complete loss of activity | Gluconobacter oxydans | |
eserine | 0.5 mM, 42% residual activity | Gluconobacter oxydans | |
paraoxon | 0.5 mM, complete loss of activity | Gluconobacter oxydans | |
Phenylglyoxal | 0.5 mM, 77% residual activity | Gluconobacter oxydans | |
pyridoxal 5'-phosphate | 0.5 mM, 62% residual activity | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
3-naphthyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
0.0079 | - |
2-naphthyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
0.0088 | - |
3-naphthyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
0.01 | - |
phenyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
0.0128 | - |
2-naphthyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
0.0174 | - |
4-nitrophenyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
0.174 | - |
4-nitrophenyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
7.2 | - |
S-methyl thiobutanoate | pH 8.0, 25° | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Gluconobacter oxydans |
76800 | - |
gel filtration | Gluconobacter oxydans |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 10% v/v, 93% residual activity | Gluconobacter oxydans |
acetonitrile | 10% v/v, 95% residual activity | Gluconobacter oxydans |
dimethyl sulfoxide | 10% v/v, 90% residual activity, 40% v/v, 45% residual activity | Gluconobacter oxydans |
Ethanol | 10% v/v, 92% residual activity | Gluconobacter oxydans |
Methanol | 10% v/v, 90% residual activity, 40% v/v, 57% residual activity | Gluconobacter oxydans |
propanol | 10% v/v, 90% residual activity | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q5FSJ2 | - |
- |
Gluconobacter oxydans 621H | Q5FSJ2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
214 | - |
pH 7.0, 25°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
Gluconobacter oxydans | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Gluconobacter oxydans 621H | 2-naphthol + acetate | - |
? | |
2-naphthyl butanoate + H2O | - |
Gluconobacter oxydans | 2-naphthol + butanoate | - |
? | |
3-naphthyl acetate + H2O | - |
Gluconobacter oxydans | 3-naphthol + acetate | - |
? | |
3-naphthyl butanoate + H2O | - |
Gluconobacter oxydans | 3-naphthol + butanoate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Gluconobacter oxydans | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Gluconobacter oxydans 621H | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Gluconobacter oxydans | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Gluconobacter oxydans 621H | 4-nitrophenol + butanoate | - |
? | |
additional information | substrate specificity of this arylesterase is higher toward short chain pnitrophenyl esters (C2 to C4) and also toward aromatic esters, such as phenyl acetate. enzyme is a serine esterase, the catalytic triad is composed of residues Ser163, Asp263, and His293 in the active site | Gluconobacter oxydans | ? | - |
? | |
additional information | substrate specificity of this arylesterase is higher toward short chain pnitrophenyl esters (C2 to C4) and also toward aromatic esters, such as phenyl acetate. enzyme is a serine esterase, the catalytic triad is composed of residues Ser163, Asp263, and His293 in the active site | Gluconobacter oxydans 621H | ? | - |
? | |
phenyl acetate + H2O | - |
Gluconobacter oxydans | phenol + acetate | - |
? | |
phenyl acetate + H2O | - |
Gluconobacter oxydans 621H | phenol + acetate | - |
? | |
S-methyl thiobutanoate + H2O | - |
Gluconobacter oxydans | methanol + thiobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
Est0881 | - |
Gluconobacter oxydans |
gox0881 | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Gluconobacter oxydans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
2-naphthyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
16.4 | - |
3-naphthyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
20 | - |
S-methyl thiobutanoate | pH 8.0, 25° | Gluconobacter oxydans | |
55.2 | - |
2-naphthyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
97.2 | - |
4-nitrophenyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
99 | - |
3-naphthyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
185 | - |
4-nitrophenyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
231 | - |
phenyl acetate | pH 7.0, 25° | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
S-methyl thiobutanoate | pH 8.0, 25° | Gluconobacter oxydans | |
540 | - |
2-naphthyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
560 | - |
4-nitrophenyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
1900 | - |
3-naphthyl butanoate | pH 7.0, 25° | Gluconobacter oxydans | |
6900 | - |
2-naphthyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
10600 | - |
4-nitrophenyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
19100 | - |
3-naphthyl acetate | pH 7.0, 25° | Gluconobacter oxydans | |
23100 | - |
phenyl acetate | pH 7.0, 25° | Gluconobacter oxydans |