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Literature summary for 3.1.1.2 extracted from

  • Wang, G.; Meng, K.; Luo, H.; Wang, Y.; Huang, H.; Shi, P.; Pan, X.; Yang, P.; Yao, B.
    Molecular cloning and characterization of a novel SGNH arylesterase from the goat rumen contents (2011), Appl. Microbiol. Biotechnol., 91, 1561-1570.
    View publication on PubMed
Search for more literature for 3.1.1.2

Activating Compound

Activating Compound Comment Organism Structure
Nonidet P-40 8.2% activation at 5% (v/v) uncultured organism
Triton X-100 11.2% activation at 5% (v/v) uncultured organism
Tween 20 4.8% activation at 5% (v/v) uncultured organism
Tween 40 16.4% activation at 5% (v/v) uncultured organism
Tween 80 19.6% activation at 5% (v/v) uncultured organism

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured organism

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 73.5% residual activity at 5 mM uncultured organism
Ag+ 9.7% residual activity at 5 mM uncultured organism
Ca2+ 88.5% residual activity at 5 mM uncultured organism
Co2+ 92.6% residual activity at 5 mM uncultured organism
Cr3+ 39.8% residual activity at 5 mM uncultured organism
Cu2+ 28.9% residual activity at 5 mM uncultured organism
diethyl dicarbonate 5.5% residual activity at 5 mM uncultured organism
dithiothreitol 19.4% residual activity at 5 mM uncultured organism
EDTA 94.2% residual activity at 5 mM uncultured organism
Fe3+ 13.7% residual activity at 5 mM uncultured organism
Hg2+ 7.2% residual activity at 5 mM uncultured organism
Mg2+ 89.2% residual activity at 5 mM uncultured organism
Mn2+ 85.4% residual activity at 5 mM uncultured organism
additional information addition of glycerin has no effect on the enzyme activity uncultured organism
NaCl 43.7% residual activity at 4 M uncultured organism
Ni2+ 86.3% residual activity at 5 mM uncultured organism
Pb2+ 45.7% residual activity at 5 mM uncultured organism
phenylmethylsulfonyl fluoride complete inhibition at 5 mM uncultured organism
SDS complete inhibition at 5% (w/v) uncultured organism
Urea 64.7% residual activity at 8 M uncultured organism
Zn2+ 76.4% residual activity at 5 mM uncultured organism

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 1-naphthyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
0.53
-
 phenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
0.96
-
 4-nitrophenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, SDS-PAGE uncultured organism
24000
-
 x * 24000, calculated from amino acid sequence uncultured organism

Organism

Organism UniProt Comment Textmining
uncultured organism G0YFD9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 75 gel filtration uncultured organism

Source Tissue

Source Tissue Comment Organism Textmining
additional information bacterium from goat rumen content uncultured organism
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O 15.4% activity compared to 4-nitrophenyl acetate uncultured organism 1-naphthol + acetate
-
 ?
4-nitrophenyl acetate + H2O highest catalytic efficiency (100% activity) uncultured organism 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O 19.7% activity compared to 4-nitrophenyl acetate uncultured organism 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O 4.1% activity compared to 4-nitrophenyl acetate uncultured organism 4-nitrophenol + caproate
-
 ?
additional information the enzyme has no activity towards 4-nitrophenyl esters with acyl chains longer than C8 like 4-nitrophenyl caprylate, 4-nitrophenyl caprate, 4-nitrophenyl palmitate, as well as 4-nitrophenyl phosphate and methyl parathion uncultured organism ?
-
 ?
phenyl acetate + H2O 166% activity compared to 4-nitrophenyl acetate uncultured organism phenol + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE uncultured organism
? x * 24000, calculated from amino acid sequence uncultured organism

Synonyms

Synonyms Comment Organism
EstR5
-
 uncultured organism
SGNH arylesterase
-
 uncultured organism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 uncultured organism

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 the enzyme exhibits more than 50% of the maximal activity between 40°C and 65°C uncultured organism

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 60 the enzyme is stable at 45°C, retaining 91.5% of the activity after 1 h incubation. At 60°C, the enzyme activity decreases rapidly and only retains 16.8% of the activity after 1 h incubation uncultured organism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 uncultured organism

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 60% of the maximum activity is retained between pH 6.0 and 9.0 uncultured organism

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 the enzyme is stable between pH 5.0 and 11.0, retaining more than 50% of the initial activity after incubation at 37°C for 1 h uncultured organism

pI Value

Organism Comment pI Value Maximum pI Value
uncultured organism calculated from amino acid sequence
-
 4.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
204.5
-
 4-nitrophenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
401.4
-
 phenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
1612
-
 1-naphthyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism