Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D183N | the mutant disfavors paraoxon binding due to its charged nature and possible electrostatic repulsion with the phosphate group of paraoxon | Homo sapiens |
F222D | the mutation abolishes both esterase and enzyme activity of PON1 | Homo sapiens |
F222Y | the mutation abolishes the PON activity of enzyme but retains the esterase activity with a 1.5fold increase in KM for phenyl acetate | Homo sapiens |
H115W | the mutant shows a 2fold increase in enzyme activity compared to the wild type enzyme | Homo sapiens |
L69V | the mutant shows a 4-16fold increase in enzyme activity compared to the wild type enzyme | Homo sapiens |
L69V/V369A | the mutant shows increased paraoxon binding affinity compared to the wild type enzyme | Homo sapiens |
R192Q | the polymorphic residue R192Q interacts with the leaving group of paraoxon, suggesting it plays an important role in the proper positioning of this substrate in the active site | Homo sapiens |
S193P | the mutation increases phosphotriesterase activity of enzyme | Homo sapiens |
V346A | the mutant shows a 4-16fold increase in enzyme activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
phenyl acetate | recombinant enzyme | Homo sapiens | |
2.3 | - |
4-acetoxybenzaldehyde | recombinant enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains Ca2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the active site of the enzyme is characterized by two distinct binding regions, the hydrophobic binding site for arylesters/lactones and the paraoxon binding site for phosphotriesters | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27169 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | the enzyme is expressed mainly in the liver | Homo sapiens | - |
serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-acetoxybenzaldehyde + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | the active site of the enzyme is characterized by two distinct binding regions, the hydrophobic binding site for arylesters/lactones and the paraoxon binding site for phosphotriesters | Homo sapiens | ? | - |
? | |
phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
4-acetoxybenzaldehyde | recombinant enzyme | Homo sapiens | |
698 | - |
phenyl acetate | recombinant enzyme | Homo sapiens |