Literature summary for 3.1.1.2 extracted from

Stevens, R.C.; Suzuki, S.M.; Cole, T.B.; Park, S.S.; Richter, R.J.; Furlong, C.E.
Engineered recombinant human paraoxonase 1 (rHuPON1) purified from Escherichia coli protects against organophosphate poisoning (2008), Proc. Natl. Acad. Sci. USA, 105, 12780-12784.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.957	-	phenyl acetate	recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P27169	-	-

Purification (Commentary)

Purification (Comment)	Organism
ceramic HA column chromatography, DEAE-Sepharose column chromatography, Sephadex G-25 gel filtration, and tert-butyl Sepharose column chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.15	-	recombinant enzyme from crude cell extract	Homo sapiens
465	-	recombinant enzyme after 90fold purification	Homo sapiens

Storage Stability

Storage Stability	Organism
4°C, purified recombinant enzyme in 20 mM Tris pH 7.7, 1 mM CaCl2, at least one month, remains stable	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phenyl acetate + H2O	-	Homo sapiens	phenol + acetate	-	?

Synonyms

Synonyms	Comment	Organism
AREase	-	Homo sapiens

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Release 2023.1 (January 2023)