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Literature summary for 3.1.1.2 extracted from
- Cloning and characterization of thermostable esterase from Archaeoglobus fulgidus (2008), J. Microbiol., 46, 100-107.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
ketoprofen ethyl ester | at pH 8.0 | Archaeoglobus fulgidus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
estimated from amino acid sequence | Archaeoglobus fulgidus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | organic solvents addition (acetone, acetonitrile, iso-propanol, ethanol, methanol, dimethyl sulfoxide, and dimethyl formamide) is not suitable for improvement of enzyme reaction through increase of substrate solubility | Archaeoglobus fulgidus |
| Triton X-100 | 1060% relative activity in the presence of 1% (v/v) Triton X-100 | Archaeoglobus fulgidus |
| Triton X-15 | 210% relative activity in the presence of 1% (v/v) Triton X-15 | Archaeoglobus fulgidus |
| Triton X-165 | 1749% relative activity in the presence of 1% (v/v) Triton X-165 | Archaeoglobus fulgidus |
| Triton X-305 | 1628% relative activity in the presence of 1% (v/v) Triton X-305 | Archaeoglobus fulgidus |
| Triton X-45 | 662% relative activity in the presence of 1% (v/v) Triton X-45 | Archaeoglobus fulgidus |
| Tween 20 | 1396% relative activity in the presence of 1% (v/v) Tween 20 | Archaeoglobus fulgidus |
| Tween 40 | 1306% relative activity in the presence of 1% (v/v) Tween 40 | Archaeoglobus fulgidus |
| Tween 60 | 1396% relative activity in the presence of 1% (v/v) Tween 60 | Archaeoglobus fulgidus |
| Tween 80 | 1339% relative activity in the presence of 1% (v/v) Tween 80 | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
strain DSM 4304 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni+2-NTA resin chromatography | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | highest specific activity | Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caprylate + H2O | 10% activity compared to 4-nitrophenyl butyrate | Archaeoglobus fulgidus | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + hexanoate | - |
? | |
| ketoprofen ethyl ester + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl laurate and 4-nitrophenyl palmitate | Archaeoglobus fulgidus | ? | - |
? | |
| additional information | the enzyme is a carboxylesterase and has highest activity with 4-nitrophenyl butyrate, activity decreases with acylglycerols containing longer carbon chains | Archaeoglobus fulgidus | ? | - |
? | |
| phenyl acetate + H2O | - |
Archaeoglobus fulgidus | phenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27500, estimated from amino acid sequence | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
| 80 | - |
using 4-nitrophenyl butyrate as substrate | Archaeoglobus fulgidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | 10% of activity is retained after 3 h at 90°C using ketoprofen ethyl ester as substrate, the enzyme shows about 50% activity at 40°C, 68% relative activity at 50°C, about 90% activity at 60°C, 100% activity at 70°C, about 90% activity at 80°C, and 58% activity at 90°C | Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
no loss of activity after 180 min at 65°C | Archaeoglobus fulgidus |
| 70 | - |
no loss of activity after 180 min at 70°C | Archaeoglobus fulgidus |
| 75 | - |
about 20% loss of activity after 180 min at 75°C | Archaeoglobus fulgidus |
| 80 | - |
about 30% loss of activity after 180 min at 80°C | Archaeoglobus fulgidus |
| 85 | - |
about 40% loss of activity after 180 min at 85°C | Archaeoglobus fulgidus |
| 90 | - |
about 50% loss of activity after 80 min at 90°C | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 12 | enzyme activity is decreased higher than pH 8.0, no activity at pH 3.0 and 12.0, about 10% relative activity is retained at pH 4 and 11 | Archaeoglobus fulgidus |
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