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Literature summary for 3.1.1.2 extracted from

  • Nus, M.; Sanchez-Muniz, F.J.; Gago, J.V.; Lopez-Oliva, E.; Sanchez-Montero, J.M.
    Determination of rat and mice arylesterase activity using serum mimetics (2008), Enzyme Microb. Technol., 43, 252-256.
No PubMed abstract available
Search for more literature for 3.1.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 phenyl acetate wild type enzyme, in simulated body fluid, at pH 7.34-7.4 and 37°C Mus musculus
0.201
-
 phenyl acetate wild type enzyme, in 20 mM Tris-HCl and 0.9 mM CaCl2, at pH 8 and 25°C Mus musculus
0.46
-
 phenyl acetate wild type enzyme, in simulated body fluid, at pH 7.34-7.4 and 37°C Rattus norvegicus
0.64
-
 phenyl acetate wild type enzyme, in 20 mM Tris-HCl and 0.9 mM CaCl2, at pH 8 and 25°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.9 mM, the further increase of the calcium concentration does not significantly increase arylesterase activity Rattus norvegicus
Ca2+ 0.9 mM, the further increase of the calcium concentration does not significantly increase arylesterase activity Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P52430
-
-
Rattus norvegicus P55159
-
-

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Rattus norvegicus
-
serum
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenyl acetate + H2O
-
 Rattus norvegicus phenol + acetate
-
 ?
phenyl acetate + H2O
-
 Mus musculus phenol + acetate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 increasing the temperature over 25°C in the Tris/HCl medium does not cause the activation of arylesterase Rattus norvegicus
25
-
 increasing the temperature over 25°C in the Tris/HCl medium does not cause the activation of arylesterase Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 diminishing the pH below 8.0 in the Tris/HCl medium does not cause the activation of arylesterase Rattus norvegicus
8
-
 diminishing the pH below 8.0 in the Tris/HCl medium does not cause the activation of arylesterase Mus musculus