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Literature summary for 3.1.1.2 extracted from

  • Aharoni, A.; Gaidukov, L.; Yagur, S.; Toker, L.; Silman, I.; Tawfik, D.S.
    Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization (2004), Proc. Natl. Acad. Sci. USA, 101, 482-487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G1A7 Homo sapiens
0.18
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G1B11 Homo sapiens
0.211
-
 2-naphthyl acetate pH 8.0 Oryctolagus cuniculus
0.26
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G2C2 Homo sapiens
0.5
-
 2-naphthyl acetate pH 8.0 Mus musculus
0.65
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G3G3 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15166
-
-
Mus musculus Q62087
-
-
Oryctolagus cuniculus Q9BGN0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
 Homo sapiens 2-naphthol + acetate
-
 ?
2-naphthyl acetate + H2O
-
 Oryctolagus cuniculus 2-naphthol + acetate
-
 ?
2-naphthyl acetate + H2O
-
 Mus musculus 2-naphthol + acetate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.66
-
 2-naphthyl acetate pH 8.0 Oryctolagus cuniculus
1.9
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G1A7 Homo sapiens
2
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G1B11 Homo sapiens
2.12
-
 2-naphthyl acetate pH 8.0 Mus musculus
18.8
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G2C2 Homo sapiens
26.25
-
 2-naphthyl acetate pH 8.0, recombinant genetic variant G3G3 Homo sapiens