Protein Variants | Comment | Organism |
---|---|---|
H115A | activity with phenyl acetate is 0.3% compared to wild-type activity, activity with 2-naphthyl acetate is 0.3% of wild-type activity | Homo sapiens |
H115Q | activity with phenyl acetate is 0.6% compared to wild-type activity, activity with 2-naphthyl acetate is 1% of wild-type activity | Homo sapiens |
H115W | activity with phenyl acetate is 0.06% compared to wild-type activity, activity with 2-naphthyl acetate is 0.3% of wild-type activity | Homo sapiens |
H134Q | activity with phenyl acetate is 9.9% compared to wild-type activity, activity with 2-naphthyl acetate is 3.1% of wild-type activity | Homo sapiens |
H184Q | activity with phenyl acetate is 32% compared to wild-type activity, activity with 2-naphthyl acetate is 66% of wild-type activity | Homo sapiens |
H184T | activity with phenyl acetate is 27% compared to wild-type activity, activity with 2-naphthyl acetate is 35% of wild-type activity | Homo sapiens |
H285Q | activity with phenyl acetate is 12% compared to wild-type activity, activity with 2-naphthyl acetate is 19% of wild-type activity | Homo sapiens |
H285S | activity with phenyl acetate is 10% compared to wild-type activity, activity with 2-naphthyl acetate is 61% of wild-type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
phenyl acetate | pH 8.0, wild-type enzyme | Homo sapiens | |
3.6 | - |
phenyl acetate | pH 8.0, mutant enzyme H134Q | Homo sapiens | |
8.1 | - |
phenyl acetate | pH 8.0, mutant enzyme H115Q | Homo sapiens | |
9.7 | - |
phenyl acetate | pH 8.0, mutant enzyme H115Q/H134Q | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | high density lipoprotein-associated enzyme | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Asp269 and Glu53 participate in the ligation of catalytic Ca2+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27169 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
Homo sapiens | 2-naphthol + acetate | - |
? | |
naphthalen-2-yl acetate + H2O | - |
Homo sapiens | 2-naphthol + acetate | - |
? | |
phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
phenyl acetate | pH 8.0, mutant enzyme H115Q/H134Q | Homo sapiens | |
10.5 | - |
phenyl acetate | pH 8.0, mutant enzyme H115Q | Homo sapiens | |
18 | - |
naphthalen-2-yl acetate | pH 8.0, wild-type enzyme | Homo sapiens | |
185 | - |
phenyl acetate | pH 8.0, mutant enzyme H134Q | Homo sapiens | |
854 | - |
phenyl acetate | pH 8.0, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-rate profile of PON1 with 2-naphthyl acetate | Homo sapiens |