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Literature summary for 3.1.1.2 extracted from

  • Khersonsky, O.; Tawfik, D.S.
    The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases (2006), J. Biol. Chem., 281, 7649-7656.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H115A activity with phenyl acetate is 0.3% compared to wild-type activity, activity with 2-naphthyl acetate is 0.3% of wild-type activity Homo sapiens
H115Q activity with phenyl acetate is 0.6% compared to wild-type activity, activity with 2-naphthyl acetate is 1% of wild-type activity Homo sapiens
H115W activity with phenyl acetate is 0.06% compared to wild-type activity, activity with 2-naphthyl acetate is 0.3% of wild-type activity Homo sapiens
H134Q activity with phenyl acetate is 9.9% compared to wild-type activity, activity with 2-naphthyl acetate is 3.1% of wild-type activity Homo sapiens
H184Q activity with phenyl acetate is 32% compared to wild-type activity, activity with 2-naphthyl acetate is 66% of wild-type activity Homo sapiens
H184T activity with phenyl acetate is 27% compared to wild-type activity, activity with 2-naphthyl acetate is 35% of wild-type activity Homo sapiens
H285Q activity with phenyl acetate is 12% compared to wild-type activity, activity with 2-naphthyl acetate is 19% of wild-type activity Homo sapiens
H285S activity with phenyl acetate is 10% compared to wild-type activity, activity with 2-naphthyl acetate is 61% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
phenyl acetate pH 8.0, wild-type enzyme Homo sapiens
3.6
-
phenyl acetate pH 8.0, mutant enzyme H134Q Homo sapiens
8.1
-
phenyl acetate pH 8.0, mutant enzyme H115Q Homo sapiens
9.7
-
phenyl acetate pH 8.0, mutant enzyme H115Q/H134Q Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information high density lipoprotein-associated enzyme Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Asp269 and Glu53 participate in the ligation of catalytic Ca2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
Homo sapiens 2-naphthol + acetate
-
?
naphthalen-2-yl acetate + H2O
-
Homo sapiens 2-naphthol + acetate
-
?
phenyl acetate + H2O
-
Homo sapiens phenol + acetate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.4
-
phenyl acetate pH 8.0, mutant enzyme H115Q/H134Q Homo sapiens
10.5
-
phenyl acetate pH 8.0, mutant enzyme H115Q Homo sapiens
18
-
naphthalen-2-yl acetate pH 8.0, wild-type enzyme Homo sapiens
185
-
phenyl acetate pH 8.0, mutant enzyme H134Q Homo sapiens
854
-
phenyl acetate pH 8.0, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-rate profile of PON1 with 2-naphthyl acetate Homo sapiens