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Literature summary for 3.1.1.2 extracted from

  • Yeung, D.T.; Josse, D.; Nicholson, J.D.; Khanal, A.; McAndrew, C.W.; Bahnson, B.J.; Lenz, D.E.; Cerasoli, D.M.
    Structure/function analyses of human serum paraoxonase (HuPON1) mutants designed from a DFPase-like homology model (2004), Biochim. Biophys. Acta, 1702, 67-77.
    View publication on PubMed
Search for more literature for 3.1.1.2

Protein Variants

Protein Variants Comment Organism
C284D no enzymic activity Homo sapiens
D269E no enzymic activity Homo sapiens
D54N no enzymic activity Homo sapiens
E313A slightly decreased activity Homo sapiens
E314A enzymic activity similar to wild-type Homo sapiens
F222D no enzymic activity Homo sapiens
F222Y increased activity with phenyl acetate, no activity with paraoxon Homo sapiens
G11A slightly enhanced activity with phenyl acetate and paraoxon Homo sapiens
G11C slightly enhanced activity with phenyl acetate and paraoxon Homo sapiens
G11S slightly enhanced activity with phenyl acetate and paraoxon Homo sapiens
H115W no enzymic activity with phenyl acetate, increased activity with paraoxon Homo sapiens
H115W/N133S no enzymic activity with phenyl acetate, slightly decreased activity with paraoxon Homo sapiens
H134W no enzymic activity Homo sapiens
H134Y no enzymic activity Homo sapiens
H285D no enzymic activity Homo sapiens
H285Y no enzymic activity Homo sapiens
L69F no enzymic activity Homo sapiens
N133S enzymic activity similar to wild-type Homo sapiens
N168E no enzymic activity Homo sapiens
N224A no enzymic activity Homo sapiens
V304A no enzymic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 phenyl acetate mutant E313A, pH 8.0, 25°C Homo sapiens
0.59
-
 phenyl acetate mutant N133S, pH 8.0, 25°C Homo sapiens
0.61
-
 phenyl acetate wild-type, pH 8.0, 25°C Homo sapiens
0.64
-
 phenyl acetate mutant G11S, pH 8.0, 25°C Homo sapiens
0.67
-
 phenyl acetate mutant E314A, pH 8.0, 25°C Homo sapiens
0.74
-
 phenyl acetate mutant G11A or mutant G11C, pH 8.0, 25°C Homo sapiens
0.89
-
 phenyl acetate mutant F222Y, pH 8.0, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens