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Literature summary for 3.1.1.14 extracted from
- The breakdown of chlorophyll by chlorophyllase (1961), Biochem. J., 78, 359-364.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | presence of a solvent such as acetone is essential for enzyme action | Beta vulgaris |  |
General Stability
| General Stability | Organism |
|---|---|
| 60% loss of activity after dialysis against distilled water in the cold for 48 h | Beta vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Beta vulgaris | first stage in the enzymic breakdown of chlorophyll in vivo may be the removal of the phytol side chain by chlorophyllase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beta vulgaris | - |
var. saccharifera | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Beta vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Beta vulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | first stage in the enzymic breakdown of chlorophyll in vivo may be the removal of the phytol side chain by chlorophyllase | Beta vulgaris | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | - |
Beta vulgaris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
45 | 0°C: about 35% of maximal activity, 45°C: about 50% of maximal activity | Beta vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
24 h, 30-50% loss of activity | Beta vulgaris |
| 45 | - |
1 h, pH 7, citrate buffer, stable. Complete loss of activity in presence of 40% acetone | Beta vulgaris |
| 60 | - |
10 min, 40% loss of activity | Beta vulgaris |
| 65 | - |
destroyed within 10 min | Beta vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | soluble enzyme | Beta vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8.7 | pH 6.4: about 70% of maximal activity, pH 8.7: about 80% of maximal activity, soluble enzyme | Beta vulgaris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
rapid inactivation | Beta vulgaris |
| 6 | 8 | 0°C, loss of 2% of activity per day | Beta vulgaris |
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Release 2023.1 (January 2023)
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