Application | Comment | Organism |
---|---|---|
synthesis | recombinant enzyme CrCLH1 can be used as a biocatalyst to produce chlorophyllide derivatives | Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
gene CLH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant C-terminally GFP-tagged enzyme expression driven by a CAMV35S promoter is determined in chloroplasts of Arabidopsis thaliana mesophyll protoplasts (isolated from 3-4-week-old well-expanded leaves yet to flower), recombinant overexpression of soluble N-terminally T7-tagged enzyme in Escherichia coli strain BL21(DE3) | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0566 | - |
chlorophyll a | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii | |
0.0999 | - |
chlorophyll b | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii | |
0.4603 | - |
bacteriochlorophyll a | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Chlamydomonas reinhardtii | - |
phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8J2S9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant T7-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacteriochlorophyll a + H2O | - |
Chlamydomonas reinhardtii | phytol + bacteriochlorophyllide a | - |
? | |
chlorophyll + H2O | - |
Chlamydomonas reinhardtii | phytol + chlorophyllide | - |
? | |
chlorophyll a + H2O | preferred substrate | Chlamydomonas reinhardtii | phytol + chlorophyllide a | - |
? | |
chlorophyll b + H2O | - |
Chlamydomonas reinhardtii | phytol + chlorophyllide b | - |
? | |
additional information | recombinant CrCLH1 has a higher catalytic efficiency for chlorophyll a than for chlorophyll b and bacteriochlorophyll a. Recombinant CrCLH1 catalyzes the conversion of chlorophyll a to pheophorbide a at pH 5.0 | Chlamydomonas reinhardtii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36500, recombinant T7-tagged enzyme, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
Chlase | - |
Chlamydomonas reinhardtii |
chlorophyllase 1 | - |
Chlamydomonas reinhardtii |
chlorophyllase1 | - |
Chlamydomonas reinhardtii |
CLH1 | - |
Chlamydomonas reinhardtii |
CrCLH1 | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Chlamydomonas reinhardtii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | recombinant enzyme, activity range, profile overview. 90% of maximal activity at 20°C and 45°C, 70% at 50°C, 25% at 70°C, inactivation at 80°C | Chlamydomonas reinhardtii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | purified recombinant enzyme, completely stable up to, 35% activity remaining at 70°C, inactivation at 80°C | Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
212.2 | - |
bacteriochlorophyll a | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii | |
232.8 | - |
chlorophyll b | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii | |
559.55 | - |
chlorophyll a | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Chlamydomonas reinhardtii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | over 50% of maximal activity within this range | Chlamydomonas reinhardtii |
5 | 7 | over 90% of maximal activity within this range | Chlamydomonas reinhardtii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | purified recombinant enzyme, stability profile, overview, over 50% activity remaining at pH 4.5-9.0, over 90% at pH 7.0, completely stable at pH 5.0-6.0 | Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme shares th highly conserved lipase motif GXSXG | Chlamydomonas reinhardtii |
physiological function | recombinant chlorophyllase 1, CrCLH1, can perform chlorophyll dephytylation and produce chlorophyllide and phytol | Chlamydomonas reinhardtii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
461 | - |
bacteriochlorophyll a | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii | |
2330 | - |
chlorophyll b | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii | |
9886 | - |
chlorophyll a | recombinant enzyme, pH 7.0, 60°C | Chlamydomonas reinhardtii |