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Literature summary for 3.1.1.14 extracted from
- Immobilization and biocatalysis of chlorophyllase in selected organic solvent systems (2005), J. Biotechnol., 120, 273-283.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | removal of green pigments from canola oil | Phaeodactylum tricornutum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Eupergit C | immobilization support | Phaeodactylum tricornutum | |
| Eupergit C/EDA | immobilization support | Phaeodactylum tricornutum | |
| refined bleached deodorized canola oil | 20% | Phaeodactylum tricornutum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0123 | - |
Chlorophyll | free enzyme in 20 mM sodium phosphate buffer pH 8 | Phaeodactylum tricornutum |  |
| 0.0186 | - |
Chlorophyll | free enzyme in PBS-hexane mixture (60/40, v/v) containing 75 mM Span85 | Phaeodactylum tricornutum | |
| 0.0189 | - |
Chlorophyll | immobilized enzyme in PBS-hexane mixture (60/40, v/v) containing 75 mM Span85 | Phaeodactylum tricornutum | |
| 0.0225 | - |
Chlorophyll | immobilized enzyme in 20 mM sodium phosphate buffer pH 8 | Phaeodactylum tricornutum | |
| 0.0245 | - |
Chlorophyll | free enzyme in PBS-hexane mixture (70/30, v/v) | Phaeodactylum tricornutum | |
| 0.0246 | - |
Chlorophyll | immobilized enzyme in PBS-hexane mixture (70/30, v/v) | Phaeodactylum tricornutum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaeodactylum tricornutum | - |
Bacillariphyceae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyll + H2O | - |
Phaeodactylum tricornutum | chlorophyllide + phytol | - |
ir | |
| pheophytin + H2O | - |
Phaeodactylum tricornutum | pheophorbide + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chlorophyll chlorophyllido-hydrolase | - |
Phaeodactylum tricornutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity and stability decrease significantly above or below pH 8.0 | Phaeodactylum tricornutum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
stability decreases significantly above or below pH 8.0 | Phaeodactylum tricornutum |
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