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Literature summary for 3.1.1.13 extracted from
- Arterial neutral cholesteryl esterase. A hormone-sensitive enzyme distinct from lysosomal cholesteryl esterase (1983), J. Biol. Chem., 258, 192-198.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP-dependent protein kinase type II | activation only for neutral CEase | Oryctolagus cuniculus | |
| dibutyryl cAMP | 2fold stimulation | Oryctolagus cuniculus |  |
| glucagon | 2fold stimulation for neutral CEase | Oryctolagus cuniculus | |
| sodium taurocholic acid | - |
Oryctolagus cuniculus | |
General Stability
| General Stability | Organism |
|---|---|
| neutral CEase stable to repeated freezing and thawing, acid CEase unstable | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sodium taurocholic acid | marked inhibition above 0.015 mM | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
cholesteryl oleate | for neutral CEase | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| lipid particle | cholesteryl ester rich droplet | Oryctolagus cuniculus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Oryctolagus cuniculus | important rate-limiting step in steroidogenesis | cholesterol + fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
rabbit | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aorta | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
for neutral CEase in presence of dibutyryl-cAMP, MgATP2- and cAMP-dependent protein kinase | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, neutral CEase, 1 month. | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | important rate-limiting step in steroidogenesis | Oryctolagus cuniculus | cholesterol + fatty acid | - |
? | |
| cholesteryl oleate + H2O | - |
Oryctolagus cuniculus | cholesterol + oleic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid cholesterol esterase | - |
Oryctolagus cuniculus |
| neutral cholesterol esterase | - |
Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
80% loss of activity for acid CEase and 25% for neutral CEase at pH 7.0 | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cAMP | activation only for neutral CEase | Oryctolagus cuniculus | |
| cAMP | as cofactor for cAMP-dependent protein kinase | Oryctolagus cuniculus | |
| MgATP2- | activation only for neutral CEase | Oryctolagus cuniculus | |
| MgATP2- | increasing activity with addition of cAMP-dependend protein kinase | Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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