Application | Comment | Organism |
---|---|---|
synthesis | enzyme shows high enantioselectivity in the kinetic resolution of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester, which produce a valuable chiral intermediate-(3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid for the drug Pregabalin | Thermomyces lanuginosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermomyces lanuginosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Thermomyces lanuginosus | |
Ca2+ | - |
Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | W8DVW0 | - |
- |
Thermomyces lanuginosus DSM 10635 | W8DVW0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester + H2O | - |
Thermomyces lanuginosus | (3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid + ethanol | - |
? | |
4-nitrophenyl acetate + H2O | about 55% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | about 55% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus DSM 10635 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | best substrate | Thermomyces lanuginosus | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl decanoate + H2O | about 10% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl decanoate + H2O | about 10% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus DSM 10635 | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | about 65% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | about 65% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus DSM 10635 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | about 30% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | about 30% of the activity with 4-nitrophenyl butanoate | Thermomyces lanuginosus DSM 10635 | 4-nitrophenol + octanoate | - |
? | |
additional information | no substrate: 4-nitrophenyl palmitate | Thermomyces lanuginosus | ? | - |
? | |
additional information | no substrate: 4-nitrophenyl palmitate | Thermomyces lanuginosus DSM 10635 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34500, calculated, x * 35000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermomyces lanuginosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
80% of maximum activtiy | Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
8 h, 50% resiudal activity | Thermomyces lanuginosus |
70 | - |
60 min, 53% residual activity | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thermomyces lanuginosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | low activity below or above | Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 12 h, 80% residual activity | Thermomyces lanuginosus |