Cloned (Comment) | Organism |
---|---|
functionally expressed in Escherichia coli as His-tagged protein | Pyrobaculum sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | 15% v/v, 1.8fold activation. 30% v/v, 32% loss of activity | Pyrobaculum sp. | |
Co2+ | 1 mM, 5% inhibition. 10 mM, 27% inhibition | Pyrobaculum sp. | |
Cu2+ | 1 mM, 15% inhibition. 10 mM, 95% inhibition | Pyrobaculum sp. | |
DMSO | 30% v/v, 22% loss of activity | Pyrobaculum sp. | |
ethanol | 15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity | Pyrobaculum sp. | |
Isopropanol | 15% v/v, 1.4fold activation. 30% v/v, 21% loss of activity | Pyrobaculum sp. | |
Mg2+ | 1 mM, 5% loss of activity | Pyrobaculum sp. | |
Mn2+ | 1 mM; 10 mM, 12% loss of activity | Pyrobaculum sp. | |
PMSF | 1 mM, 28% loss of activity | Pyrobaculum sp. | |
SDS | 0.1% v/v, 17% loss of activity. 1% v/v, 68% loss of activity | Pyrobaculum sp. | |
Tween-20 | 0.1% v/v, 16% loss of activity. 1% v/v, 33% loss of activity | Pyrobaculum sp. | |
Zn2+ | 1 mM, 8% inhibition. 10 mM, 49% inhibition | Pyrobaculum sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
4-nitrophenyl acetate | pH 8.0, 80°C | Pyrobaculum sp. | |
1.27 | - |
4-nitrophenyl butyrate | pH 8.0, 80°C | Pyrobaculum sp. | |
2.64 | - |
4-nitrophenyl octanoate | pH 8.0, 80°C | Pyrobaculum sp. | |
4.99 | - |
4-nitrophenyl decanoate | pH 8.0, 80°C | Pyrobaculum sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
acetone | 15% or 30% v/v, 1.2fold activation | Pyrobaculum sp. | |
acetonitrile | 15% v/v, 1.8fold activation. 30% v/v, 32% loss of activity | Pyrobaculum sp. | |
Ca2+ | 10 mM, 10 mM, 1.1fold activation | Pyrobaculum sp. | |
CTAB | 0.1% v/v, 1.4fold activation. 1% v/v, 1.8fold activation | Pyrobaculum sp. | |
ethanol | 15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity | Pyrobaculum sp. | |
Isopropanol | 15% v/v, 1.4fold activation. 30% v/v, 21% loss of activity | Pyrobaculum sp. | |
K+ | 10 mM, 1.2fold activation | Pyrobaculum sp. | |
methanol | 15% v/v, 1.4fold activation. 30% v/v, 1.7fold activation | Pyrobaculum sp. | |
Mg2+ | 10 mM, 1.2fold activation | Pyrobaculum sp. | |
Na+ | 10 mM, 1.1fold activation | Pyrobaculum sp. | |
Triton X-100 | 0.1% v/v, 1.3fold activation. 1% v/v, 31% loss of activity | Pyrobaculum sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21130 | - |
calculated from sequence | Pyrobaculum sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum sp. | G7VG08 | - |
- |
Pyrobaculum sp. 1860 | G7VG08 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Pyrobaculum sp. | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pyrobaculum sp. 1860 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pyrobaculum sp. | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pyrobaculum sp. 1860 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Pyrobaculum sp. | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Pyrobaculum sp. 1860 | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Pyrobaculum sp. | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Pyrobaculum sp. 1860 | 4-nitrophenol + octanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P186_1588 | - |
Pyrobaculum sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pyrobaculum sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 40°C: about 50% of maximal activity, 90°C: 80% of maximal activity | Pyrobaculum sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
circular dichroism results indicated that rP186_1588 shows slight structure alteration from 60 to 90 °C. Comparative molecular simulations at different temperatures (27°C, 80°C, 100°C and 200°C) reveal that its thermostability is associated with its conformational rigidity | Pyrobaculum sp. |
90 | - |
6 h, the enzyme retains 70% of relative activity | Pyrobaculum sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.19 | - |
4-nitrophenyl decanoate | pH 8.0, 80°C | Pyrobaculum sp. | |
6.36 | - |
4-nitrophenyl octanoate | pH 8.0, 80°C | Pyrobaculum sp. | |
9.12 | - |
4-nitrophenyl butyrate | pH 8.0, 80°C | Pyrobaculum sp. | |
11.65 | - |
4-nitrophenyl acetate | pH 8.0, 80°C | Pyrobaculum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pyrobaculum sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 50% of maxiomal activity, pH 10.0: about 60% of maximal activity | Pyrobaculum sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrobaculum sp. | calculated | - |
6.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.24 | - |
4-nitrophenyl decanoate | pH 8.0, 80°C | Pyrobaculum sp. | |
2.41 | - |
4-nitrophenyl octanoate | pH 8.0, 80°C | Pyrobaculum sp. | |
7.18 | - |
4-nitrophenyl butyrate | pH 8.0, 80°C | Pyrobaculum sp. | |
33.29 | - |
4-nitrophenyl acetate | pH 8.0, 80°C | Pyrobaculum sp. |