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Literature summary for 3.1.1.1 extracted from

  • Xie, Z.; Xu, B.; Ding, J.; Liu, L.; Zhang, X.; Li, J.; Huang, Z.
    Heterologous expression and characterization of a malathion-hydrolyzing carboxylesterase from a thermophilic bacterium, Alicyclobacillus tengchongensis (2013), Biotechnol. Lett., 35, 1283-1289.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information the addition of 1% (v/v) acetone and 1% (v/v) ethanol has little effect on enzyme activity Alicyclobacillus tengchongensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Alicyclobacillus tengchongensis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition at 1 mM Alicyclobacillus tengchongensis
Cu2+ strong inhibition at 1 mM Alicyclobacillus tengchongensis
Hg2+ strong inhibition at 1 mM Alicyclobacillus tengchongensis
SDS strong inhibition at 1% (v/v) Alicyclobacillus tengchongensis
Zn2+ strong inhibition at 1 mM Alicyclobacillus tengchongensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 2-naphthyl acetate at pH 7.0 and 25°C Alicyclobacillus tengchongensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates at 1 mM Alicyclobacillus tengchongensis
Pb2+ activates at 1 mM Alicyclobacillus tengchongensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57820
-
 x * 57820, calculated from amino acid sequence Alicyclobacillus tengchongensis
58000
-
 x * 58000, SDS-PAGE Alicyclobacillus tengchongensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malathion + H2O Alicyclobacillus tengchongensis i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate ?
-
 ?
malathion + H2O Alicyclobacillus tengchongensis D-1 i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Alicyclobacillus tengchongensis S4TYB2
-
-
Alicyclobacillus tengchongensis D-1 S4TYB2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Alicyclobacillus tengchongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
 Alicyclobacillus tengchongensis 2-naphthol + acetate
-
 ?
2-naphthyl acetate + H2O
-
 Alicyclobacillus tengchongensis D-1 2-naphthol + acetate
-
 ?
malathion + H2O i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate Alicyclobacillus tengchongensis ?
-
 ?
malathion + H2O i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate Alicyclobacillus tengchongensis D-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Alicyclobacillus tengchongensis
? x * 57820, calculated from amino acid sequence Alicyclobacillus tengchongensis

Synonyms

Synonyms Comment Organism
D1CarE5
-
 Alicyclobacillus tengchongensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Alicyclobacillus tengchongensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 more than 50% activity between 30 and 70°C Alicyclobacillus tengchongensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 the enzyme remains stable at 25°C for 50 min and loses about 25% and 65% of its activity after 50 min at 37°C and 60°C, respectively Alicyclobacillus tengchongensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Alicyclobacillus tengchongensis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.3 more than 50% activity between pH 5.5 and 7.3 Alicyclobacillus tengchongensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.4 8.9 the enzyme remains stable at pH 7.0 for 50 min and loses 20% and all of its activity after 50 min at pH 8.0 and 5.4, respectively Alicyclobacillus tengchongensis

pI Value

Organism Comment pI Value Maximum pI Value
Alicyclobacillus tengchongensis calculated from amino acid sequence
-
 4.5