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Literature summary for 3.1.1.1 extracted from

  • Xiang, D.F.; Kumaran, D.; Swaminathan, S.; Raushel, F.M.
    Structural characterization and function determination of a nonspecific carboxylate esterase from the amidohydrolase superfamily with a promiscuous ability to hydrolyze methylphosphonate esters (2014), Biochemistry, 53, 3476-3485.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cereibacter sphaeroides

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M MgCl2, and 0.1 M Tris, pH 8.5 Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
E175K the mutant shows 10fold reduced Mn2+ content compared to the wild type enzyme Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
4-methylumbelliferyl heptanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.033
-
4-nitrophenyl octanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.048
-
4-nitrophenyl hexanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.056
-
4-nitrophenyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.11
-
paraoxon in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.16
-
4-Methylumbelliferyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.18
-
1-naphthyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.22
-
ethyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.24
-
4-methylumbelliferyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.33
-
ethyl butyrylacetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.4
-
2-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.42
-
methyl paraoxon in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.42
-
ethyl 4-nitrophenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.52
-
4-nitrophenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.56
-
2-naphthyl propionate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.62
-
ethyl 2-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.64
-
1-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.65
-
4-nitroacetanilide in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.7
-
isobutyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.9
-
1,4-diacetoxybenzene in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.2
-
4-methylbenzyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.2
-
isopropyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.6
-
Benzyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.6
-
4'-chloroacetanilide in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.8
-
3-acetoxypyridine in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.9
-
indoxyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.8
-
phenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains iron Cereibacter sphaeroides
Mn2+ contains Mn2+ Cereibacter sphaeroides
Zn2+ contains Zn2+ Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography and Superdex 200 gel filtration Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-diacetoxybenzene + H2O
-
Cereibacter sphaeroides ?
-
?
1-naphthyl acetate + H2O
-
Cereibacter sphaeroides 1-naphthol + acetate
-
?
1-naphthyl butyrate + H2O
-
Cereibacter sphaeroides 1-naphthol + butyrate
-
?
2-naphthyl acetate + H2O
-
Cereibacter sphaeroides 2-naphthol + acetate
-
?
2-naphthyl propionate + H2O
-
Cereibacter sphaeroides 2-naphthol + propionate
-
?
2-phenethyl acetate + H2O
-
Cereibacter sphaeroides 2-phenylethanol + acetate
-
?
2-thionaphthyl acetate + H2O
-
Cereibacter sphaeroides 2-thionaphthol + acetate
-
?
3-acetoxypyridine + H2O
-
Cereibacter sphaeroides ?
-
?
4'-chloroacetanilide + H2O
-
Cereibacter sphaeroides ?
-
?
4-hydroxyphenyl acetate + H2O
-
Cereibacter sphaeroides 4-hydroxyphenol + acetate
-
?
4-methylbenzyl acetate + H2O
-
Cereibacter sphaeroides 4-methylbenzyl alcohol + acetate
-
?
4-methylumbelliferyl acetate + H2O
-
Cereibacter sphaeroides 4-methylumbelliferone + acetate
-
?
4-methylumbelliferyl acetate + H2O
-
Cereibacter sphaeroides 4-methylumbelliferol + acetate
-
?
4-methylumbelliferyl butyrate + H2O
-
Cereibacter sphaeroides 4-methylumbelliferone + butyrate
-
?
4-methylumbelliferyl heptanoate + H2O
-
Cereibacter sphaeroides 4-methylumbelliferone + heptanoate
-
?
4-nitroacetanilide + H2O
-
Cereibacter sphaeroides ?
-
?
4-nitrophenyl acetate + H2O
-
Cereibacter sphaeroides 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Cereibacter sphaeroides 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O best substrate Cereibacter sphaeroides 4-nitrophenol + butyrate
-
?
4-nitrophenyl hexanoate + H2O
-
Cereibacter sphaeroides 4-nitrophenol + hexanoate
-
?
4-nitrophenyl methylphosphonate + H2O
-
Cereibacter sphaeroides 4-nitrophenol + methylphosphonate
-
?
4-nitrophenyl octanoate + H2O
-
Cereibacter sphaeroides 4-nitrophenol + octanoate
-
?
benzyl acetate + H2O
-
Cereibacter sphaeroides benzyl alcohol + acetate
-
?
cyclohexyl 4-nitrophenyl methylphosphonate + H2O
-
Cereibacter sphaeroides ?
-
?
ethyl (4-nitrophenyl)acetate + H2O
-
Cereibacter sphaeroides ethanol + (4-nitrophenyl)acetate
-
?
ethyl (naphthalen-2-yl)acetate + H2O
-
Cereibacter sphaeroides (naphthalen-2-yl)acetate + ethanol
-
?
ethyl 4-nitrophenyl methylphosphonate + H2O
-
Cereibacter sphaeroides ?
-
?
ethyl butyrylacetate + H2O
-
Cereibacter sphaeroides ?
-
?
indoxyl acetate + H2O
-
Cereibacter sphaeroides indol-3-ol + acetate
-
?
isobutyl 4-nitrophenyl methylphosphonate + H2O
-
Cereibacter sphaeroides ?
-
?
isobutyl 4-nitrophenyl methylphosphonate + H2O
-
Cereibacter sphaeroides 4-nitrophenol + isobutyl methylphosphonate
-
?
isopropyl 4-nitrophenyl methylphosphonate + H2O
-
Cereibacter sphaeroides ?
-
?
methyl paraoxon + H2O
-
Cereibacter sphaeroides ?
-
?
paraoxon + H2O
-
Cereibacter sphaeroides diethylphosphate + 4-nitrophenol
-
?
phenyl acetate + H2O
-
Cereibacter sphaeroides phenol + acetate
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
Rsp3690
-
Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.063
-
4-methylumbelliferyl heptanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.1
-
4'-chloroacetanilide in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.36
-
4-nitroacetanilide in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.5
-
4-nitrophenyl octanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.65
-
4-methylumbelliferyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.79
-
ethyl butyrylacetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.3
-
paraoxon in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.7
-
ethyl 2-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.8
-
ethyl 4-nitrophenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.1
-
methyl paraoxon in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.3
-
indoxyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.5
-
Benzyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
3.1
-
isopropyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
4.3
-
3-acetoxypyridine in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
4.8
-
1-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
4.9
-
4-Methylumbelliferyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
6.5
-
4-nitrophenyl hexanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
8
-
isobutyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
9
-
1-naphthyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
30
-
phenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
40
-
2-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
47
-
4-methylbenzyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
50
-
4-nitrophenyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
83
-
2-naphthyl propionate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
84
-
ethyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
108
-
1,4-diacetoxybenzene in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
120
-
4-nitrophenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.063
-
4'-chloroacetanilide in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.21
-
cyclohexyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.25
-
2-phenethyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.47
-
2-thionaphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
0.55
-
4-nitroacetanilide in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
1.2
-
indoxyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.4
-
3-acetoxypyridine in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.4
-
ethyl butyrylacetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.6
-
4-methylumbelliferyl heptanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.6
-
isopropyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.7
-
4-methylumbelliferyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
2.8
-
ethyl 2-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
4.3
-
ethyl 4-nitrophenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
5
-
methyl paraoxon in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
7.5
-
1-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
11
-
phenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
11
-
isobutyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
12
-
paraoxon in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
15
-
4-nitrophenyl octanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
31
-
4-Methylumbelliferyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
38
-
4-methylbenzyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
100
-
2-naphthyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
130
-
4-nitrophenyl hexanoate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
130
-
1,4-diacetoxybenzene in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
150
-
2-naphthyl propionate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
160
-
Benzyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
230
-
4-nitrophenyl acetate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
380
-
ethyl 4-nitrophenyl methylphosphonate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
500
-
1-naphthyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides
880
-
4-nitrophenyl butyrate in 2.5 mM Bicine buffer, pH 8.3, at 30°C Cereibacter sphaeroides