Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M HEPES/MOPS-Na, pH 7.5, 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.03 M MgCl2, and 0.03 M CaCl2 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
D287A | the mutant shows about 81fold increase in activity and significant decrease in enantioselectivity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate compared to the wild type | Pseudomonas putida |
W187H | the mutant shows about 100fold increase in activity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate compared to the wild type | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ECU1011 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rac-2-acetoxy-2-(2'-chlorophenyl) acetate + H2O | - |
Pseudomonas putida | ? | - |
? | |
rac-2-acetoxy-2-(2'-chlorophenyl) acetate + H2O | - |
Pseudomonas putida ECU1011 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
esterase | - |
Pseudomonas putida |
PPE | - |
Pseudomonas putida |