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Literature summary for 3.1.1.1 extracted from

  • Dou, S.; Kong, X.D.; Ma, B.D.; Chen, Q.; Zhang, J.; Zhou, J.; Xu, J.H.
    Crystal structures of Pseudomonas putida esterase reveal the functional role of residues 187 and 287 in substrate binding and chiral recognition (2014), Biochem. Biophys. Res. Commun., 446, 1145-1150.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M HEPES/MOPS-Na, pH 7.5, 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.03 M MgCl2, and 0.03 M CaCl2 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
D287A the mutant shows about 81fold increase in activity and significant decrease in enantioselectivity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate compared to the wild type Pseudomonas putida
W187H the mutant shows about 100fold increase in activity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate compared to the wild type Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ECU1011
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rac-2-acetoxy-2-(2'-chlorophenyl) acetate + H2O
-
Pseudomonas putida ?
-
?
rac-2-acetoxy-2-(2'-chlorophenyl) acetate + H2O
-
Pseudomonas putida ECU1011 ?
-
?

Synonyms

Synonyms Comment Organism
esterase
-
Pseudomonas putida
PPE
-
Pseudomonas putida