Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | some increase for the activity of the wild-type enzyme treated with acetone | Aeropyrum pernix |
Protein Variants | Comment | Organism |
---|---|---|
R11G/L36P/V225A/I551L/A564T | in the resolution of 2-octanol acetate, the acetone-treated mutant A has a 9fold enantioselective increase relative to that purified by Ni-chelating column | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | inhibition of mutant enzyme R11G/L36P/V225A/I551L/A564T no matter that it is or is not treated by acetone | Aeropyrum pernix | |
Tween-80 | inhibition of wild-type and mutant enzyme R11G/L36P/V225A/I551L/A564T no matter that they are or are not treated by acetone | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YBQ2 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YBQ2 | - |
- |
Purification (Comment) | Organism |
---|---|
acetone is utilized to purify wild-type and mutant enzymes to improve their activity and enantioselectivity | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-octanyl acetate + H2O | the mutant enzyme R11G/L36P/V225A/I551L/A564T treated twice with acetone has a 9fold increase for enantioselectivity in resolution of 2-octanyl acetate relative to that purified by Ni-chelating column, but the wild-type enzyme is less sensitive to acetone in terms of enantioselectivity | Aeropyrum pernix | 2-octanol + acetate | - |
? | |
2-octanyl acetate + H2O | the mutant enzyme R11G/L36P/V225A/I551L/A564T treated twice with acetone has a 9fold increase for enantioselectivity in resolution of 2-octanyl acetate relative to that purified by Ni-chelating column, but the wild-type enzyme is less sensitive to acetone in terms of enantioselectivity | Aeropyrum pernix DSM 11879 | 2-octanol + acetate | - |
? | |
4-nitrophenyl octanoate + H2O | acetone is utilized to purify wild-type and mutant enzymes to improve their activity and enantioselectivity | Aeropyrum pernix | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | acetone is utilized to purify wild-type and mutant enzymes to improve their activity and enantioselectivity | Aeropyrum pernix DSM 11879 | 4-nitrophenol + octanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1547 | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
- |
Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
acetone treatment shifts the optimum pH of wild-type and mutant enzymes | Aeropyrum pernix |
8 | - |
assay at | Aeropyrum pernix |
8 | - |
wild-type enzyme purified by Ni-chelating column | Aeropyrum pernix |
8.3 | - |
mutant enzyme R11G/L36P/V225A/I551L/A564T purified by Ni-chelating column | Aeropyrum pernix |
9 | - |
wild-type enzyme purified by acetone treatment, mutant enzyme R11G/L36P/V225A/I551L/A564T purified by acetone treatment | Aeropyrum pernix |