Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D266N | specific activity of the mutant enzyme is 2% of the wild-type activity | Saccharolobus solfataricus |
H295N | specific activity of the mutant enzyme is 2% of the wild-type activity | Saccharolobus solfataricus |
S144A | specific activity of the mutant enzyme is 1% of the wild-type activity | Saccharolobus solfataricus |
S144C | specific activity of the mutant enzyme is 5% of the wild-type activity | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethylfluorophosphate | 0.01 mM, 30°C, 30 min, 75% inhibition | Saccharolobus solfataricus | |
diphenylamine-2-carboxylate | 5 mM, 30°C, 30 min, 77% inhibition | Saccharolobus solfataricus | |
eserine | 5 mM, 30°C, 30 min, 18% inhibition | Saccharolobus solfataricus | |
paraoxon | 5 mM, 30°C, 30 min, 90% inhibition | Saccharolobus solfataricus | |
PCMB | 5 mM, 30°C, 30 min, 72% inhibition | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
4-nitrophenyl decanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
0.029 | - |
4-nitrophenyl octanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
0.037 | - |
4-nitrophenyl dodecanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
0.051 | - |
4-nitrophenyl hexanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
0.13 | - |
4-nitrophenyl hexadecanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
0.222 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require a divalent cation for activity | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34260 | - |
1 * 34260, calculated from sequence | Saccharolobus solfataricus |
35000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 90%, 30°C, 30 min, stable | Saccharolobus solfataricus |
Ethanol | 90%, 30°C, 30 min, stable | Saccharolobus solfataricus |
Methanol | 90%, 30°C, 30 min, stable | Saccharolobus solfataricus |
SDS | 5%, 30°C, 30 min, about 70% loss of activity | Saccharolobus solfataricus |
urea | 8 M, 30°C, 30 min, about 80% loss of activity | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | M5DDN2 | - |
- |
Saccharolobus solfataricus P1 | M5DDN2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1392 | - |
pH 8.0, 60°C, purified enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl dodecanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl dodecanoate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl hexadecanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + octanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Saccharolobus solfataricus |
monomer | 1 * 34260, calculated from sequence | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | 70°C: about 55% of maximal activity, 90°C: about 80% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
120 h, stable | Saccharolobus solfataricus |
80 | - |
120 h, 70% loss of activity | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
861 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C | Saccharolobus solfataricus | |
1138 | - |
4-nitrophenyl hexadecanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
1312 | - |
4-nitrophenyl dodecanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
1476 | - |
4-nitrophenyl hexanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
1491 | - |
4-nitrophenyl octanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
2337 | - |
4-nitrophenyl decanoate | pH 8.0, 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3900 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C | Saccharolobus solfataricus | |
8700 | - |
4-nitrophenyl hexadecanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
29100 | - |
4-nitrophenyl hexanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
35900 | - |
4-nitrophenyl dodecanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
51800 | - |
4-nitrophenyl octanoate | pH 8.0, 60°C | Saccharolobus solfataricus | |
97400 | - |
4-nitrophenyl decanoate | pH 8.0, 60°C | Saccharolobus solfataricus |