Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.1 extracted from

  • Zhou, X.; Wang, H.; Zhang, Y.; Gao, L.; Feng, Y.
    Alteration of substrate specificities of thermophilic alpha/beta hydrolases through domain swapping and domain interface optimization (2012), Acta Biochim. Biophys. Sin., 44, 965-973.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of chimeras of a carboxylesterase (EC 3.1.1.1) from Archaeoglobus fulgidus and an acylpeptide hydrolase (EC 3.4.19.1) from Aeropyrum pernix K1 Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeras of a carboxylesterase (EC 3.1.1.1) from Archaeoglobus fulgidus and an acylpeptide hydrolase (EC 3.4.19.1) from Aeropyrum pernix K1. Their activities to hydrolyze 4-nitrophenyl esters (pNP) with different acyl chain lengths is explored. The chimeras inherit the thermophilic property of both parents. The substrate-binding domain is the dominant factor on enzyme substrate specificity, and the optimization of the newly formed domain interface is an important guarantee for successful domain swapping of proteins with low-sequence homology Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0327
-
 4-nitrophenyl butanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
0.0768
-
 4-nitrophenyl acetate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
0.289
-
 4-nitrophenyl octanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
0.507
-
 4-nitrophenyl hexadecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
0.651
-
 4-nitrophenyl decanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
0.654
-
 4-nitrophenyl dodecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
1.054
-
 4-nitrophenyl tetradecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of chimeras of a carboxylesterase (EC 3.1.1.1) from Archaeoglobus fulgidus and an acylpeptide hydrolase (EC 3.4.19.1) from Aeropyrum pernix K1 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl decanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl dodecanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexadecanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + hexadecanoate
-
 ?
4-nitrophenyl octanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl tetradecanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + tetradecanoate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 wild-type enzyme Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: about 55% of maximal activity, 90°C: about 85% of maximal activity, wild-type enzyme Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3
-
 4-nitrophenyl hexadecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
42.3
-
 4-nitrophenyl tetradecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
120
-
 4-nitrophenyl acetate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
128
-
 4-nitrophenyl dodecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
277
-
 4-nitrophenyl butanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
477
-
 4-nitrophenyl decanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
831
-
 4-nitrophenyl octanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 wild-type enzyme Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 pH 6.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, wild-type enzyme Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 4-nitrophenyl hexadecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
40
-
 4-nitrophenyl tetradecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
200
-
 4-nitrophenyl dodecanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
700
-
 4-nitrophenyl decanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
1600
-
 4-nitrophenyl acetate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
2900
-
 4-nitrophenyl octanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus
8500
-
 4-nitrophenyl butanoate 80°C, pH 8.0, wild-type enzyme Archaeoglobus fulgidus