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Literature summary for 3.1.1.1 extracted from

  • Chung, Y.; Park, C.; Lee, S.
    Partial purification and characterization of thermostable esterase from the hyperthermophilic archaeon Sulfolobus solfataricus (2000), Biotechnol. Bioprocess Eng., 5, 53-56.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dimethylformamide 5% (v/v), about 1.1fold activation Saccharolobus solfataricus
ethanol 5% (v/v), about 1.2fold activation Saccharolobus solfataricus
methanol 5-20% (v/v), about 1.2fold activation Saccharolobus solfataricus

Application

Application Comment Organism
synthesis the enzyme shows chiral resolution activity for (S)-ibuprofen, indicating that the enzyme can be used for the production of commercially important chiral drugs Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol stable at high concentrations of polar organic solvents, such as ethanol and methanol as compared to other mesophilic counterparts Saccharolobus solfataricus
Methanol stable at high concentrations of polar organic solvents, such as ethanol and methanol as compared to other mesophilic counterparts Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus DSM 1617
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.5
-
pH 7.0, 75°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-ibuprofen methyl ester + H2O the enzyme has S-form specificity for ibuprofen Saccharolobus solfataricus (S)-ibuprofen + methanol
-
?
(S)-ibuprofen methyl ester + H2O the enzyme has S-form specificity for ibuprofen Saccharolobus solfataricus DSM 1617 (S)-ibuprofen + methanol
-
?
4-methylumbelliferyl acetate + H2O
-
Saccharolobus solfataricus 4-methylumbelliferone + acetate
-
?
4-methylumbelliferyl acetate + H2O
-
Saccharolobus solfataricus DSM 1617 4-methylumbelliferone + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Saccharolobus solfataricus DSM 1617 4-nitrophenol + butyrate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
110 min, about 10% loss of activity Saccharolobus solfataricus
95
-
half-life: 45 min Saccharolobus solfataricus
100
-
half-life: 15 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
4.8 9.2 pH 4.8: about 40% of maximal activity, pH 9.2: about 40% of maximal activity Saccharolobus solfataricus