Cloned (Comment) | Organism |
---|---|
overexpression system in Escherichia coli | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic)acid | - |
Archaeoglobus fulgidus | |
diethyldicarbonate | 1.5 mM, 94% inhibition | Archaeoglobus fulgidus | |
diisopropyl fluorophosphate | irreversible | Archaeoglobus fulgidus | |
phenylmethylsulfonyl fluoride | 0.08 mM, 97% inhibition | Archaeoglobus fulgidus | |
physostigmine | 0.1 mM, 96% inhibition | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
4-nitrophenyl hexanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
0.02 | - |
4-nitrophenyl pentanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
0.021 | - |
4-nitrophenyl butyrate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
0.106 | - |
4-nitrophenyl propionate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
0.119 | - |
4-nitrophenyl octanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33800 | - |
1 * 33800, SDS-PAGE | Archaeoglobus fulgidus |
43000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28558 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3000 | - |
80°C, pH 7.1, substrate: 4-nitrophenyl hexanoate | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33800, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
AFEST | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | 50°C: about 50% of maximal activity, 100°C: about 90% of maximal activity | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
t1/2: 30 h | Archaeoglobus fulgidus |
70 | - |
t1/2: 7.5 h | Archaeoglobus fulgidus |
85 | - |
t1/2: 60 min | Archaeoglobus fulgidus |
90 | - |
t1/2: 28 min | Archaeoglobus fulgidus |
95 | - |
t1/2: 26 min | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
348 | - |
4-nitrophenyl propionate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
680 | - |
4-nitrophenyl butyrate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
770 | - |
4-nitrophenyl octanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
866 | - |
4-nitrophenyl pentanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
1014 | - |
4-nitrophenyl hexanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Archaeoglobus fulgidus |
7.1 | - |
assay at | Archaeoglobus fulgidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3300 | - |
4-nitrophenyl propionate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
6500 | - |
4-nitrophenyl octanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
32400 | - |
4-nitrophenyl butyrate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
43300 | - |
4-nitrophenyl pentanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus | |
92200 | - |
4-nitrophenyl hexanoate | 70°C, pH 7.1 | Archaeoglobus fulgidus |