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Literature summary for 3.1.1.1 extracted from
- A novel esterase Sso2518 from Sulfolobus solfataricus with a much lower temperature optimum than the growth temperature (2010), Biotechnol. Lett., 32, 1103-1108.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli with fusion His-tags at the C-terminus | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 2 mM, 10% inhibition | Saccharolobus solfataricus |  |
| Ca2+ | 2 mM, 13% inhibition | Saccharolobus solfataricus | |
| Co2+ | 2 mM, 7% inhibition | Saccharolobus solfataricus | |
| DTT | 5 mM, 75% inhibition | Saccharolobus solfataricus | |
| PMSF | 5 mM, complete inhibition | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
4-nitrophenyl caproate | pH and temperature not specified in the publication | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 2 mM, enhances the enzyme activity to 134% compared to the activity of EDTA-treated enzyme | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
3 * 40000, SDS-PAGE | Saccharolobus solfataricus |
| 40400 | - |
3 * 40400, calculated from sequence | Saccharolobus solfataricus |
| 120000 | - |
native PAGE | Saccharolobus solfataricus |
| 125000 | - |
gel filtration | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97VU1 | - |
- |
| Saccharolobus solfataricus P2 | Q97VU1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caproate + H2O | the enzyme shows the highest activity with p-nitrophenyl caproate | Saccharolobus solfataricus | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caproate + H2O | the enzyme shows the highest activity with p-nitrophenyl caproate | Saccharolobus solfataricus P2 | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + laurate | - |
? | |
| additional information | the activity of the enzyme is strongly influenced by the chain length of the acyl group. Activity increases up to C6 and then decreases | Saccharolobus solfataricus | ? | - |
? | |
| additional information | the activity of the enzyme is strongly influenced by the chain length of the acyl group. Activity increases up to C6 and then decreases | Saccharolobus solfataricus P2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 40000, SDS-PAGE | Saccharolobus solfataricus |
| homotrimer | 3 * 40400, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| esterase Sso2518 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | 40°C: about 70% of maximal activity, 80°C: about 60% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half-life: 30 min | Saccharolobus solfataricus |
| 90 | - |
30 min, about 80% loss of activity | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 8.0: about 90% of maximal activity, less than 10% of maximal activity at pH 8.5 | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | calculated from sequence | - |
5.4 |
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