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Literature summary for 3.1.1.1 extracted from

  • Souleyre, E.J.; Marshall, S.D.; Oakeshott, J.G.; Russell, R.J.; Plummer, K.M.; Newcomb, R.D.
    Biochemical characterisation of MdCXE1, a carboxylesterase from apple that is expressed during fruit ripening (2011), Phytochemistry, 72, 564-571.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
CXE1 is expressed in Escherichia coli M15 cells Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
diethyl coumarylphosphate
-
 Malus domestica
paraoxon
-
 Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000053
-
 4-methylumbelliferyl palmitate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
0.000086
-
 4-methylumbelliferyl laurate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
0.00031
-
 4-methylumbelliferyl octanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
0.000618
-
 4-methylumbelliferyl heptanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
0.0227
-
 4-Methylumbelliferyl butyrate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
0.0828
-
 4-yethylumbelliferyl acetate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
 x * 39800, predicted molecular mass for the N-terminal His6-tagged version of CXE1 Malus domestica
40000
-
 x * 40000, SDS-PAGE Malus domestica

Organism

Organism UniProt Comment Textmining
Malus domestica
-
 cultivar Royal Gala AO3
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Malus domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
 Malus domestica 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl butyrate + H2O
-
 Malus domestica 4-methylumbelliferone + butyrate
-
 ?
4-methylumbelliferyl heptanoate + H2O
-
 Malus domestica 4-methylumbelliferone + heptanoate
-
 ?
4-methylumbelliferyl laurate + H2O best substrate Malus domestica 4-methylumbelliferone + laurate
-
 ?
4-methylumbelliferyl octanoate + H2O
-
 Malus domestica 4-methylumbelliferone + octanoate
-
 ?
4-methylumbelliferyl palmitate + H2O
-
 Malus domestica 4-methylumbelliferone + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Malus domestica
? x * 39800, predicted molecular mass for the N-terminal His6-tagged version of CXE1 Malus domestica

Synonyms

Synonyms Comment Organism
CXE1
-
 Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Malus domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 the activity of CXE1 declines by approximately 3fold by 50°C compared to the optimum at 35°C Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.254
-
 4-methylumbelliferyl palmitate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
2 3.7 4-yethylumbelliferyl acetate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
4.66
-
 4-methylumbelliferyl octanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
6.38
-
 4-methylumbelliferyl laurate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
6.94
-
 4-methylumbelliferyl heptanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
69.5
-
 4-Methylumbelliferyl butyrate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
 Malus domestica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000019
-
 in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica paraoxon
0.0573
-
 in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica diethyl coumarylphosphate

Expression

Organism Comment Expression
Malus domestica in wild type, gene CXE1 is expressed at low levels during early stages of fruit development, rising to a peak of expression in apple fruit at harvest maturity. It is not significantly up-regulated by ethylene in the skin of AO3 fruit. Gene CXE16 is expressed constitutively in wild type throughout fruit development, and is up-regulated by ethylene in skin of AO3 fruit for 18 h up

General Information

General Information Comment Organism
physiological function CXE1 has an impact on apple flavour through its ability to hydrolyse relevant flavour esters in ripe apple fruit Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
287
-
 4-yethylumbelliferyl acetate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
3070
-
 4-Methylumbelliferyl butyrate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
4760
-
 4-methylumbelliferyl palmitate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
11200
-
 4-methylumbelliferyl heptanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
15100
-
 4-methylumbelliferyl octanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica
74100
-
 4-methylumbelliferyl laurate in 25 mM Tris-HCl (pH 8.0), at 25°C Malus domestica