Literature summary for 3.1.1.1 extracted from

Lv, X.; Guo, L.; Song, L.; Fu, Q.; Zhao, K.; Li, A.; Luo, X.; Lu, W.
Purification and characterization of a novel extracellular carboxylesterase from the moderately halophilic bacterium Thalassobacillus sp. strain DF-E4 (2011), Ann. Microbiol., 61, 281-290.

No PubMed abstract available

Search for more literature for 3.1.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
beta-mercaptoethanol	145% activity at 5 mM	Thalassobacillus sp.
additional information	enzyme activity is not affected by SDS, Tween 20 and Tween 80 (0.2% (v/v) each)	Thalassobacillus sp.
NaCl	optimum activity occurs at 0.5 M NaCl (w/v) with 116% activity	Thalassobacillus sp.
Triton X-100	120% activity at 0.2% (v/v)	Thalassobacillus sp.

General Stability

General Stability	Organism
the enzyme is quite stable up to 4 M NaCl and retains 90% of the original activity even after 12 h incubation	Thalassobacillus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	76% residual activity at 1 mM	Thalassobacillus sp.
diethyl 4-nitrophenyl phosphate	complete inhibition at 5 mM	Thalassobacillus sp.
diethyl dicarbonate	8% residual activity at 5 mM	Thalassobacillus sp.
Fe2+	51% residual activity at 1 mM	Thalassobacillus sp.
Fe3+	23% residual activity at 1 mM	Thalassobacillus sp.
additional information	not inhibited by 1 mM EDTA and beta-mercaptoethanol	Thalassobacillus sp.
Phenylarsine oxide	10% residual activity at 5 mM	Thalassobacillus sp.
phenylmethylsulfonyl fluoride	complete inhibition at 1 mM	Thalassobacillus sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.69	-	4-nitrophenyl butyrate	pH 8.5, 40°C	Thalassobacillus sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Thalassobacillus sp.	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	enzyme activity is not affected or slightly inhibited by Na+, K+, Mg2+, Ca2+, Mn2+, Co2+, Hg2+ and Zn2+ (1 mM each)	Thalassobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	1 * 45000, SDS-PAGE	Thalassobacillus sp.
49000	-	analytical ultracentrifugation	Thalassobacillus sp.

Organic Solvent Stability

Organic Solvent	Comment	Organism
acetonitrile	82.8% activity at 10% (v/v)	Thalassobacillus sp.
dimethylformamide	74% activity at 10% (v/v)	Thalassobacillus sp.
DMSO	55.9% activity at 10% (v/v)	Thalassobacillus sp.
Ethanol	75.9% activity at 10% (v/v)	Thalassobacillus sp.
Methanol	80.7% activity at 10% (v/v)	Thalassobacillus sp.

Organism

Organism	UniProt	Comment	Textmining
Thalassobacillus sp.	-	-	-
Thalassobacillus sp. DF-E4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ultrafiltration, DEAE-Sephadex A-25 gel filtration and Sephadex G-75 gel filtration	Thalassobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
252	-	crude extract, using 4-nitrophenyl butyrate as substrate, at pH 8.5 and 40°C	Thalassobacillus sp.
2046	-	after 8.11fold purification, using 4-nitrophenyl butyrate as substrate, at pH 8.5 and 40°C	Thalassobacillus sp.

Storage Stability

Storage Stability	Organism
4°C, 50 mM Tris-HCl buffer (pH 8.0), at least 4 weeks, the enzyme remains stable	Thalassobacillus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-naphthyl acetate + H2O	-	Thalassobacillus sp.	1-naphthol + acetate	-	?
1-naphthyl acetate + H2O	-	Thalassobacillus sp. DF-E4	1-naphthol + acetate	-	?
2-naphthyl acetate + H2O	-	Thalassobacillus sp.	2-naphthol + acetate	-	?
2-naphthyl acetate + H2O	-	Thalassobacillus sp. DF-E4	2-naphthol + acetate	-	?
4-nitrophenyl acetate + H2O	89% activity compared to 4-nitrophenyl butyrate	Thalassobacillus sp.	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	89% activity compared to 4-nitrophenyl butyrate	Thalassobacillus sp. DF-E4	4-nitrophenol + acetate	-	?
4-nitrophenyl butyrate + H2O	most efficient hydrolysis with 4-nitrophenyl butyrate	Thalassobacillus sp.	4-nitrophenol + butyrate	-	?
4-nitrophenyl butyrate + H2O	most efficient hydrolysis with 4-nitrophenyl butyrate	Thalassobacillus sp. DF-E4	4-nitrophenol + butyrate	-	?
4-nitrophenyl decanoate + H2O	14% activity compared to 4-nitrophenyl butyrate	Thalassobacillus sp.	4-nitrophenol + decanoate	-	?
4-nitrophenyl hexanoate + H2O	78% activity compared to 4-nitrophenyl butyrate	Thalassobacillus sp.	4-nitrophenol + hexanoate	-	?
4-nitrophenyl octanoate + H2O	46% activity compared to 4-nitrophenyl butyrate	Thalassobacillus sp.	4-nitrophenol + octanoate	-	?
additional information	no activity on 4-nitrophenyl ester substrates with an acyl chain length greater than C12. The enzyme has no activity towards casein or trioleoylglycerol	Thalassobacillus sp.	?	-	?
additional information	no activity on 4-nitrophenyl ester substrates with an acyl chain length greater than C12. The enzyme has no activity towards casein or trioleoylglycerol	Thalassobacillus sp. DF-E4	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 45000, SDS-PAGE	Thalassobacillus sp.

Synonyms

Synonyms	Comment	Organism
LIP4	-	Thalassobacillus sp.
serine-type carboxylesterase	-	Thalassobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Thalassobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	LIP4 is very active between 30 and 50°C with an optimal temperature of 40°C, and about 32 and 71% activity at 20 and 30°C, respectively	Thalassobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	80	LIP4 is stable at temperatures below 45°C and loses about 36% of its activity when incubated for 60 min at 50°C, while the enzyme is completely inactivated at 80°C. The half-life of the enzyme is 103 min at 50°C, 36 min at 55°C, and 9 min at 60°C without any additive	Thalassobacillus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Thalassobacillus sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	9.5	the enzyme activity is stable at pH 6.0-9.5 for 1 h	Thalassobacillus sp.

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Release 2023.1 (January 2023)