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Literature summary for 3.1.1.1 extracted from

  • Oboh, O.T.; Lamango, N.S.
    Liver prenylated methylated protein methyl esterase is the same enzyme as Sus scrofa carboxylesterase (2008), J. Biochem. Mol. Toxicol., 22, 51-62.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
(3S)-1-chloro-3-tosylamido-7-amino-2-heptanone 73.6% residual activity at 1 mM Sus scrofa
asulam 91.7% residual activity at 1 mM Sus scrofa
benzil 38% residual activity at 1 mM Sus scrofa
ebelactone A inhibits more than 80% of the enzyme at 1 mM Sus scrofa
ebelactone B complete inhibition at 1 mM Sus scrofa
L-4-tosylamino-2-phenylethyl chloromethyl ketone inhibits more than 80% of the enzyme at 1 mM Sus scrofa
L-Leu-chloromethyl ketone 60.4% residual activity at 1 mM Sus scrofa
additional information not inhibited by benzyl carbamate Sus scrofa
N-(methoxysuccinyl)-L-Ala-L-Ala-L-Pro-L-Val chloromethyl ketone 61.8% residual activity at 1 mM Sus scrofa
N-alpha-tosyl-L-Lys chloromethyl ketone 98.6% residual activity at 1 mM Sus scrofa
N-carbobenzyloxy-L-Phe chloromethyl ketone 57% residual activity at 1 mM Sus scrofa
paraoxon complete inhibition at 1 mM Sus scrofa
phenylmethylsulfonyl fluoride complete inhibition at 1 mM Sus scrofa
tosyl-L-phenylalanine chloromethyl ketone inhibits more than 80% of the enzyme at 1 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 hydrocinnamoyl farnesyl-L-cysteine methyl ester
-
 Sus scrofa
0.033
-
 benzoyl-Gly-farnesyl-L-cysteine methyl ester
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE ion-exchange column chromatography, Q-Sepharose column chromatography, phenyl Sepharose column chromatography, Superdex 200 gel filtration, and Cu2+-charged immobilized metal ion affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0004
-
 crude extract Sus scrofa
0.00529
-
 after 13.23fold purification Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Sus scrofa 4-nitrophenol + acetate
-
 ?
benzoyl-Gly-farnesyl-L-cysteine methyl ester + H2O
-
 Sus scrofa ?
-
 ?
hydrocinnamoyl farnesyl-L-cysteine methyl ester + H2O
-
 Sus scrofa N-(3-phenylpropanoyl)-S-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]-L-cysteine + methanol
-
 ?

Synonyms

Synonyms Comment Organism
PPMTase
-
 Sus scrofa
prenylated methylated protein methyl esterase
-
 Sus scrofa