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Literature summary for 3.1.1.1 extracted from

  • Xie, G.; Liu, M.; Zhu, H.; Lei, B.
    Esterase SeE of Streptococcus equi ssp. equi is a novel nonspecific carboxylic ester hydrolase (2008), FEMS Microbiol. Lett., 289, 181-186.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Streptococcus equi

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate complete inhibition at 0.01 mM Streptococcus equi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 Acetylsalicylic acid in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
29
-
 tripropionin in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
56
-
 ethyl acetate in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
68
-
 vinyl propionate in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
203
-
 Triacetin in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi

Organism

Organism UniProt Comment Textmining
Streptococcus equi B5THG6 subspecies Streptococcus equi equi
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography Streptococcus equi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
 using tributyrin as substrate, at 25°C Streptococcus equi
24
-
 using vinyl butyrate as substrate, at 25°C Streptococcus equi
44
-
 using acetylsalicyclic acid as substrate, at 25°C Streptococcus equi
68
-
 using ethyl acetate as substrate, at 25°C Streptococcus equi
149
-
 using tripropionin as substrate, at 25°C Streptococcus equi
513
-
 using triacetin as substrate, at 25°C Streptococcus equi
815
-
 using vinyl propionate as substrate, at 25°C Streptococcus equi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Streptococcus equi 4-nitrophenol + acetate
-
 ?
acetylsalicylic acid + H2O
-
 Streptococcus equi salicylic acid + acetate
-
 ?
ethyl acetate + H2O
-
 Streptococcus equi ethanol + acetate
-
 ?
additional information does not hydrolyze ethyl butyrate, trioctanoin, vinyl laurate, acetylcholine, and triglycerides Streptococcus equi ?
-
 ?
triacetin + H2O
-
 Streptococcus equi acetic acid + glycerol
-
 ?
tributyrin + H2O
-
 Streptococcus equi butanoate + glycerol
-
 ?
tripropionin + H2O
-
 Streptococcus equi propionic acid + glycerol
-
 ?
vinyl butyrate + H2O
-
 Streptococcus equi vinyl alcohol + butyrate
-
 ?
vinyl propionate + H2O
-
 Streptococcus equi vinyl alcohol + propionate
-
 ?

Synonyms

Synonyms Comment Organism
SeE
-
 Streptococcus equi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptococcus equi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the reaction rates dramatically slow down with time at 50 and 60°C Streptococcus equi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptococcus equi

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the activities of SeE after 1 h incubation at pH 4 and 5 are less than 20% of the activity of SeE after 1 h incubation at pH 7, whereas only a slight decrease is observed after incubation at pH 8 and 9 Streptococcus equi