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Literature summary for 3.1.1.1 extracted from

  • Sehgal, A.C.; Callen, W.; Mathur, E.J.; Short, J.M.; Kelly, R.M.
    Carboxylesterase from Sulfolobus solfataricus P1. (2001), Methods Enzymol., 330, 461-471.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 4-yethylumbelliferyl acetate 80°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, calculated from sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
 fragment
-
Saccharolobus solfataricus P1
-
 fragment
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl acetate + H2O
-
 Saccharolobus solfataricus P1 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl butyrate + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + butyrate
-
 ?
4-methylumbelliferyl butyrate + H2O
-
 Saccharolobus solfataricus P1 4-methylumbelliferone + butyrate
-
 ?
p-nitrophenyl caproate + H2O
-
 Saccharolobus solfataricus p-nitrophenol + caproate
-
 ?
p-nitrophenyl caproate + H2O
-
 Saccharolobus solfataricus P1 p-nitrophenol + caproate
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
Sso P1 carboxylesterase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
97
-
 melting temperature Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1000
-
 4-yethylumbelliferyl acetate 80°C Saccharolobus solfataricus
1000
-
 4-yethylumbelliferyl acetate 80°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity, substrate: 4-methylumbelliferyl butyrate Saccharolobus solfataricus