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Literature summary for 3.1.1.1 extracted from
- EstA from Arthrobacter nitroguajacolicus Rue61a, a thermo- and solvent-tolerant carboxylesterase related to class C beta-lactamases (2007), Curr. Microbiol., 54, 230-236.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, His-tag | Paenarthrobacter nitroguajacolicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Paenarthrobacter nitroguajacolicus |  |
| Hg2+ | - |
Paenarthrobacter nitroguajacolicus | |
| phenylmethylsulfonyl fluoride | - |
Paenarthrobacter nitroguajacolicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
4-nitrophenyl acetate | pH 8.0, 22°C | Paenarthrobacter nitroguajacolicus | |
| 0.38 | - |
4-nitrophenyl butanoate | pH 8.0, 22°C | Paenarthrobacter nitroguajacolicus | |
| 4 | - |
phenyl acetate | pH 8.0, 22°C | Paenarthrobacter nitroguajacolicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42100 | - |
2 * 42100, calculated, His-tagged protein | Paenarthrobacter nitroguajacolicus |
| 95000 | - |
gel filtration, His-tagged protein | Paenarthrobacter nitroguajacolicus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 30%, 130% residual activity | Paenarthrobacter nitroguajacolicus |
| acetonitrile | 30%, 120% residual activity | Paenarthrobacter nitroguajacolicus |
| chloroform | 5%, complete loss of activity | Paenarthrobacter nitroguajacolicus |
| diethyl ether | 30%, 124% residual activity | Paenarthrobacter nitroguajacolicus |
| dimethyl sulfoxide | 30%, 110% residual activity | Paenarthrobacter nitroguajacolicus |
| dodecane | 5%, complete loss of activity | Paenarthrobacter nitroguajacolicus |
| Ethanol | 30%, 90% residual activity | Paenarthrobacter nitroguajacolicus |
| hexadecane | 5%, 51% residual activity | Paenarthrobacter nitroguajacolicus |
| hexane | 5%, complete loss of activity | Paenarthrobacter nitroguajacolicus |
| Methanol | 30%, 115% residual activity | Paenarthrobacter nitroguajacolicus |
| N,N-dimethylformamide | 30%, 108% residual activity | Paenarthrobacter nitroguajacolicus |
| octane | 5%, complete loss of activity | Paenarthrobacter nitroguajacolicus |
| propanol | 30%, 104% residual activity | Paenarthrobacter nitroguajacolicus |
| toluene | 5%, complete loss of activity | Paenarthrobacter nitroguajacolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter nitroguajacolicus | - |
family VIII esterase | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Paenarthrobacter nitroguajacolicus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Paenarthrobacter nitroguajacolicus | 4-nitrophenol + butanoate | - |
? | |
| additional information | enzyme catalyzed the hydrolysis of short-chain phenylacylesters and triglycerides, and shows weak activity toward 2-hydroxy- and 2-nitroanilide | Paenarthrobacter nitroguajacolicus | ? | - |
? | |
| phenyl acetate + H2O | - |
Paenarthrobacter nitroguajacolicus | phenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 2 * 42100, calculated, His-tagged protein | Paenarthrobacter nitroguajacolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Paenarthrobacter nitroguajacolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
19 days, 90% residual activity in phosphate buffer, pH 7.5, 78% residual activity in Tris/HCl buffer, pH 7.5 | Paenarthrobacter nitroguajacolicus |
| 50 | - |
19 days, 53% residual activity | Paenarthrobacter nitroguajacolicus |
| 60 | - |
24 h, 30% residual activity | Paenarthrobacter nitroguajacolicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 550 | - |
phenyl acetate | pH 8.0, 22°C | Paenarthrobacter nitroguajacolicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Paenarthrobacter nitroguajacolicus |
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