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Literature summary for 3.1.1.1 extracted from

  • Maruyama, K.; Akita, K.; Naitou, C.; Yoshida, M.; Kitamura, T.
    Purification and characterization of an esterase hydrolyzing monoalkyl phthalates from Micrococcus sp. YGJ1 (2005), J. Biochem., 137, 27-32.
    View publication on PubMed
Search for more literature for 3.1.1.1

General Stability

General Stability Organism
in absence of 2-mercaptoethanol, purified enzymes are rapidly inactivated at 25°C Micrococcus sp.
unstable in crude extract, 10% v/v ethylene glycol and 10 mM 2-mercaptoethanol or 1 mM dithiothreitol stabilize Micrococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 23% residual activity, isoform E1, 10% residual activity, isoform E2 Micrococcus sp.
diethyldicarbonate 1 mM, 1.6% residual activity, isoform E1, 2.4% residual activity, isoform E2 Micrococcus sp.
ethanol inhibitory above 10% v/V Micrococcus sp.
HgCl2 complete inhibition Micrococcus sp.
additional information not inhibitory: EDTA, 2,2’-dipyridyl, 1,10-phenanthroline, 8-hydroxyquinoline, Tiron, at 1 mM each Micrococcus sp.
p-chloromercuribenzoate complete inhibition Micrococcus sp.
phenylmethylsulfonyl fluoride 1 mM, 79% residual activity, isoform E1, 70% residual activity, isoform E2 Micrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.39
-
 monopentyl phthalate isoform E1, pH 7.5, 30°C Micrococcus sp.
1.67
-
 monobutyl phthalate isoform E1, pH 7.5, 30°C Micrococcus sp.
2.63
-
 monopentyl phthalate isoform E2, pH 7.5, 30°C Micrococcus sp.
2.7
-
 monobutyl phthalate isoform E2, pH 7.5, 30°C Micrococcus sp.
5.88
-
 monopropyl phthalate isoform E1, pH 7.5, 30°C Micrococcus sp.
6.25
-
 monopropyl phthalate isoform E2, pH 7.5, 30°C Micrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE, both E1 and E2 Micrococcus sp.
60000
-
 gel filtration, both E1 and E2 Micrococcus sp.

Organism

Organism UniProt Comment Textmining
Micrococcus sp.
-
 isoforms E1 and E2
-
Micrococcus sp. YGJ1
-
 isoforms E1 and E2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyl benzoate + H2O at 5-6% of the rate of monobutyl phthalate Micrococcus sp. benzoate + butanol
-
 ?
butyl benzoate + H2O at 5-6% of the rate of monobutyl phthalate Micrococcus sp. YGJ1 benzoate + butanol
-
 ?
monobutyl phthalate + H2O
-
 Micrococcus sp. phthalate + butanol
-
 ?
monobutyl phthalate + H2O
-
 Micrococcus sp. YGJ1 phthalate + butanol
-
 ?
monopentyl phthalate + H2O at 50% of the rate of monobutyl phthalate Micrococcus sp. phthalate + pentanol
-
 ?
monopentyl phthalate + H2O at 50% of the rate of monobutyl phthalate Micrococcus sp. YGJ1 phthalate + pentanol
-
 ?
monopropyl phthalate + H2O at 12-19% of the rate of monobutyl phthalate Micrococcus sp. phthalate + propanol
-
 ?
monopropyl phthalate + H2O at 12-19% of the rate of monobutyl phthalate Micrococcus sp. YGJ1 phthalate + propanol
-
 ?
additional information no substrate: 1-naphthyl acetate Micrococcus sp. ?
-
 ?
additional information no substrate: 1-naphthyl acetate Micrococcus sp. YGJ1 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE, both E1 and E2 Micrococcus sp.
More N-terminal amino acid sequence is identical for E1 and E2 Micrococcus sp.

Synonyms

Synonyms Comment Organism
MAP esterase
-
 Micrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 both E1 and E2 Micrococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
27 55 50% of maximum activity within this range, both E1 and E2 Micrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 both E1 and E2 Micrococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.8 50% of maximum activity within this range, both E1 and E2 Micrococcus sp.