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Literature summary for 3.1.1.1 extracted from

  • Wu, P.C.; Liu, Y.H.; Wang, Z.Y.; Zhang, X.Y.; Li, H.; Liang, W.Q.; Luo, N.; Hu, J.M.; Lu, J.Q.; Luan, T.G.; Cao, L.X.
    Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112 (2006), J. Agric. Food Chem., 54, 836-842.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Klebsiella sp.
expression in Escherichia coli Klebsiella sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.5 mM, complete inhibition; strong Klebsiella sp.
Hg2+ 0.5 mM, complete inhibition; strong Klebsiella sp.
additional information 1 mM EDTA or 1 mM phenanthroline show little effect on activity Klebsiella sp.
p-chloromercuribenzoate 0.1 mM, strong inhibition; strong Klebsiella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
 trans-permethrin pH 7.0, 30°C Klebsiella sp.
0.00016
-
 cis-permethrin pH 7.0, 30°C Klebsiella sp.
0.00021
-
 cypermethrin pH 7.0, 30°C Klebsiella sp.
0.00087
-
 malathion pH 7.0, 30°C Klebsiella sp.
0.00091
-
 fenvalerate pH 7.0, 30°C Klebsiella sp.
0.00123
-
 deltamethrin pH 7.0, 30°C Klebsiella sp.
0.037
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.
0.037
-
 4-nitrophenyl caproate pH 7.0, 30°C Klebsiella sp.
0.068
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
0.068
-
 4-nitrophenyl caprylate pH 7.0, 30°C Klebsiella sp.
0.092
-
 4-nitrophenyl butyrate pH 7.0, 30°C Klebsiella sp.
0.092
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
0.139
-
 4-nitrophenyl propionate pH 7.0, 30°C Klebsiella sp.
0.139
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
0.261
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
0.261
-
 p-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
0.339
-
 4-nitrophenyl laurate pH 7.0, 30°C Klebsiella sp.
0.339
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 gel filtration Klebsiella sp.
73000
-
 gel filtration Klebsiella sp.
73500
-
 1 * 73000, SDS-PAGE, 1 * 73500, calculated Klebsiella sp.

Organism

Organism UniProt Comment Textmining
Klebsiella sp. Q52NW7
-
-
Klebsiella sp. Q52NW7 strain ZD112
-
Klebsiella sp. ZD112 Q52NW7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
 substrate p-nitrophenyl deltamethrin, pH 7.0, 30°C Klebsiella sp.
2.4
-
 substrate p-nitrophenyl malathion, pH 7.0, 30°C Klebsiella sp.
3.5
-
 substrate p-nitrophenyl fenvalerate, pH 7.0, 30°C Klebsiella sp.
9.8
-
 substrate p-nitrophenyl cypermethrin, pH 7.0, 30°C Klebsiella sp.
35.6
-
 substrate p-nitrophenyl cis-permethrin, pH 7.0, 30°C Klebsiella sp.
38.8
-
 substrate p-nitrophenyl trans-permethrin, pH 7.0, 30°C Klebsiella sp.
67
-
 substrate p-nitrophenyl laurate, pH 7.0, 30°C Klebsiella sp.
91
-
 substrate p-nitrophenyl acetate, pH 7.0, 30°C Klebsiella sp.
246.6
-
 substrate: 4-nitrophenyl acetate, pH 7.0, 30°C Klebsiella sp.
269
-
 substrate p-nitrophenyl propionate, pH 7.0, 30°C Klebsiella sp.
355
-
 substrate p-nitrophenyl butyrate, pH 7.0, 30°C Klebsiella sp.
407
-
 substrate p-nitrophenyl caprylate, pH 7.0, 30°C Klebsiella sp.
573
-
 substrate p-nitrophenyl caproate, pH 7.0, 30°C Klebsiella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Klebsiella sp. 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Klebsiella sp. ZD112 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Klebsiella sp. 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Klebsiella sp. 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Klebsiella sp. ZD112 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O
-
 Klebsiella sp. 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caprylate + H2O
-
 Klebsiella sp. 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl dodecanoate + H2O medium-chain 4-nitrophenyl esters are the preferred substrates, whereas 4-nitrophenyl esters of longer chain fatty acids are poor substrates Klebsiella sp. 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexanoate + H2O medium-chain 4-nitrophenyl esters are the preferred substrates, whereas 4-nitrophenyl esters of longer chain fatty acids are poor substrates Klebsiella sp. 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl laurate + H2O
-
 Klebsiella sp. 4-nitrophenol + laurate
-
 ?
4-nitrophenyl octanoate + H2O medium-chain 4-nitrophenyl esters are the preferred substrates, whereas 4-nitrophenyl esters of longer chain fatty acids are poor substrates Klebsiella sp. 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl propanoate + H2O
-
 Klebsiella sp. 4-nitrophenol + propanoate
-
 ?
4-nitrophenyl propionate + H2O
-
 Klebsiella sp. 4-nitrophenol + propionate
-
 ?
4-nitrophenyl propionate + H2O
-
 Klebsiella sp. ZD112 4-nitrophenol + propionate
-
 ?
cis-permethrin + H2O
-
 Klebsiella sp. ?
-
 ?
cypermethrin + H2O
-
 Klebsiella sp. ?
-
 ?
deltamethrin + H2O
-
 Klebsiella sp. 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
fenvalerate + H2O
-
 Klebsiella sp. ?
-
 ?
malathion + H2O
-
 Klebsiella sp. ?
-
 ?
additional information no substrate: 4-nitrophenyl myristate, 4-nitrophenol + palmitate Klebsiella sp. ?
-
 ?
additional information no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate Klebsiella sp. ?
-
 ?
additional information no substrate: 4-nitrophenyl myristate, 4-nitrophenol + palmitate Klebsiella sp. ZD112 ?
-
 ?
additional information no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate Klebsiella sp. ZD112 ?
-
 ?
trans-permethrin + H2O
-
 Klebsiella sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 73000, SDS-PAGE Klebsiella sp.
monomer 1 * 73000, SDS-PAGE, 1 * 73500, calculated Klebsiella sp.

Synonyms

Synonyms Comment Organism
EstP
-
 Klebsiella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate: 4-nitrophenyl acetate Klebsiella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 1 h, pH 7.0, stable up to Klebsiella sp.
50
-
 1 h, pH 7.0, 46% loss of activity Klebsiella sp.
65
-
 1 h, pH 7.0, complete inactivation Klebsiella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 deltamethrin pH 7.0, 30°C Klebsiella sp.
0.07
-
 malathion pH 7.0, 30°C Klebsiella sp.
0.103
-
 fenvalerate pH 7.0, 30°C Klebsiella sp.
0.288
-
 cypermethrin pH 7.0, 30°C Klebsiella sp.
1.05
-
 cis-permethrin pH 7.0, 30°C Klebsiella sp.
1.14
-
 trans-permethrin pH 7.0, 30°C Klebsiella sp.
59
-
 4-nitrophenyl laurate pH 7.0, 30°C Klebsiella sp.
59
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.
81
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
81
-
 p-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
238
-
 4-nitrophenyl propionate pH 7.0, 30°C Klebsiella sp.
238
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
314
-
 4-nitrophenyl butyrate pH 7.0, 30°C Klebsiella sp.
314
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
360
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
360
-
 4-nitrophenyl caprylate pH 7.0, 30°C Klebsiella sp.
507
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.
507
-
 4-nitrophenyl caproate pH 7.0, 30°C Klebsiella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate: 4-nitrophenyl acetate Klebsiella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 2 h, 30°C, stable Klebsiella sp.

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella sp. isoelectric focusing, pH-range: 3.5-10.0
-
 8.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.
310
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
1710
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
3410
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
5300
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
13700
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.