Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | slight activation | Pseudomonas fluorescens | |
Triton X-100 | 11fold increased activity | Pseudomonas fluorescens | |
Triton X-15 | 3.3fold increased activity | Pseudomonas fluorescens | |
Triton X-165 | 9.5fold increased activity | Pseudomonas fluorescens | |
Triton X-305 | 10.5fold increased activity | Pseudomonas fluorescens | |
Triton X-45 | 9.4fold increased activity | Pseudomonas fluorescens | |
Tween 20 | 1.7fold increased activity | Pseudomonas fluorescens | |
Tween 40 | 6.3fold increased activity | Pseudomonas fluorescens | |
Tween 60 | 6.2fold increased activity | Pseudomonas fluorescens | |
Tween 80 | 6.6fold increased activity | Pseudomonas fluorescens |
Application | Comment | Organism |
---|---|---|
biotechnology | engineered enzyme can be used to produce the enantiomer (S)-ketoprofen for pharmacological or pharmaceutical use | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
genetic analysis, expression in Escherichia coli as N-terminally His-tagged enzyme | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | slight inhibition | Pseudomonas fluorescens | |
acetone | slight inhibition | Pseudomonas fluorescens | |
Ag2+ | strong inhibition | Pseudomonas fluorescens | |
butanol | strong inhibition | Pseudomonas fluorescens | |
ethanol | - |
Pseudomonas fluorescens | |
Fe2+ | strong inhibition | Pseudomonas fluorescens | |
Hg2+ | strong inhibition | Pseudomonas fluorescens | |
additional information | no inhibition by methanol | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | 45000 | recombinant enzyme, gel filtration | Pseudomonas fluorescens |
42000 | - |
1 * 42000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | Q8VU79 | - |
- |
Pseudomonas fluorescens KCTC1767 | Q8VU79 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77 | - |
purified recombinant enzyme, substrate 4-nitrophenyl palmitate | Pseudomonas fluorescens |
387 | - |
purified recombinant enzyme, substrate 4-nitrophenyl caprylate | Pseudomonas fluorescens |
2598 | - |
purified recombinant enzyme, substrate 4-nitrophenyl butyrate | Pseudomonas fluorescens |
5242 | - |
purified recombinant enzyme, substrate 4-nitrophenyl acetate | Pseudomonas fluorescens |
27000 | - |
purified recombinant enzyme, substrate (S)-ketoprofen ethyl ester | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-ketoprofen ethyl ester + H2O | enantioselective activity, best substrate | Pseudomonas fluorescens | (S)-ketoprofen + ethanol | - |
? | |
(S)-ketoprofen ethyl ester + H2O | enantioselective activity, best substrate | Pseudomonas fluorescens KCTC1767 | (S)-ketoprofen + ethanol | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas fluorescens KCTC1767 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pseudomonas fluorescens KCTC1767 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Pseudomonas fluorescens KCTC1767 | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl palmitate + H2O | low activity | Pseudomonas fluorescens | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | low activity | Pseudomonas fluorescens KCTC1767 | 4-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 35 | stable | Pseudomonas fluorescens |
40 | - |
2 h, loss of 50% activity | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | 10 | - |
Pseudomonas fluorescens |