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Literature summary for 3.1.1.1 extracted from
- The EstA esterase is responsible for the main capacity of Lactococcus lactis to synthesize short chain fatty acid esters in vitro (2002), J. Appl. Microbiol., 93, 994-1002.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, genetic organization, expression in Escherichia coli | Lactococcus lactis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of negative mutant strains, showing reduced activity, by gene replacement for modification of the active site | Lactococcus lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | Lactococcus lactis | - |
4-nitrophenol + butyrate | - |
? |  |
| ethanol + acetate | Lactococcus lactis | ester formation | ethyl acetate + H2O | - |
? | |
| ethanol + acetyl-CoA | Lactococcus lactis | ester formation | ethyl acetate + CoA | - |
? | |
| ethanol + butyrate | Lactococcus lactis | ester formation | ethyl butanoate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | Q8KWI9 | ssp. cremoris, strain CNRZ253, EstA esterase | - |
| Lactococcus lactis | Q8KWI9 | ssp. lactis, strain TIL46, EstA esterase | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
ester synthesizing activity | Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Lactococcus lactis | 4-nitrophenol + butyrate | - |
? | |
| ethanol + acetate | ester formation | Lactococcus lactis | ethyl acetate + H2O | - |
? | |
| ethanol + acetyl-CoA | ester formation | Lactococcus lactis | ethyl acetate + CoA | - |
? | |
| ethanol + butyrate | ester formation | Lactococcus lactis | ethyl butanoate + H2O | - |
? | |
| methanethiol + acetyl-CoA | ester formation | Lactococcus lactis | methanethioacetate + coenzyme A | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EstA esterase | - |
Lactococcus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lactococcus lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
ester synthesis | Lactococcus lactis |
| 8 | - |
assay at | Lactococcus lactis |
| 8 | - |
assay at, ester hydrolysis | Lactococcus lactis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 10fold reduced activity at pH 5.0 and 4fold reduced activity at pH 8.0 compared to maximal activity at pH 7.0 | Lactococcus lactis |
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