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Literature summary for 3.1.1.1 extracted from

  • Nishizawa, M.; Gomi, H.; Kishimoto, F.
    Purification and some properties of carboxylesterase from Arthrobacter globiformis stereoselective hydrolysis of ethyl chrysanthemate (1993), Biosci. Biotechnol. Biochem., 57, 594-598.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
DFP
-
Arthrobacter globiformis
eserine eserine sulfate Arthrobacter globiformis
PMSF
-
Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Arthrobacter globiformis
94000
-
gel filtration Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis SC-69828
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter globiformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.8
-
-
Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Arthrobacter globiformis 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Arthrobacter globiformis SC-69828 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Arthrobacter globiformis 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Arthrobacter globiformis SC-69828 4-nitrophenol + butyrate
-
?
4-nitrophenyl chrysanthemate + H2O
-
Arthrobacter globiformis 4-nitrophenol + chrysanthemate
-
?
4-nitrophenyl hexanoate + H2O
-
Arthrobacter globiformis 4-nitrophenol + hexanoate
-
?
4-nitrophenyl hexanoate + H2O
-
Arthrobacter globiformis SC-69828 4-nitrophenol + hexanoate
-
?
4-nitrophenyl octanoate + H2O
-
Arthrobacter globiformis 4-nitrophenol + octanoate
-
?
4-nitrophenyl octanoate + H2O
-
Arthrobacter globiformis SC-69828 4-nitrophenol + octanoate
-
?
4-nitrophenyl propionate + H2O
-
Arthrobacter globiformis 4-nitrophenol + propionate
-
?
4-nitrophenyl propionate + H2O
-
Arthrobacter globiformis SC-69828 4-nitrophenol + propionate
-
?
phenolphthalein dibutyrate + H2O
-
Arthrobacter globiformis ?
-
?
triacetin + H2O
-
Arthrobacter globiformis acetic acid + glycerol
-
?
tributyrylglycerol + H2O
-
Arthrobacter globiformis butyrate + glycerol
-
?
tricaproin + H2O
-
Arthrobacter globiformis caproic acid + glycerol
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Arthrobacter globiformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 35°C: about 45% of maximal activity, 55°C: about 60% of maximal activity Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
24 h, 13% loss of activity Arthrobacter globiformis
50
-
24 h, 39% loss of activity Arthrobacter globiformis
60
-
30 min, stable Arthrobacter globiformis
75
-
loss of activity at or above Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
hydrolysis of phenolphthalein dibutyrate Arthrobacter globiformis

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 pH 8.0: about 35% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of phenolphthalein dibutyrate Arthrobacter globiformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 stable Arthrobacter globiformis